缺乏理想亲本细胞系是阻碍人单克隆抗体淋巴细胞杂交瘤技术发展的重要因素之一。作者以肾综合征出血热患者外用血单个核细胞(PBMC)为统一B淋巴细胞来源,对目前常用的4株融合用亲本细胞系的融合率以及它们所构建的杂交瘤细胞分泌人 IgM,人IgG的比例进行了比较研究。发现人淋巴母细胞系 HF2、小鼠骨髓瘤细胞系 Ag8.653、人-鼠骨髓瘤细胞系 SHM-D33和杂交骨髓瘤细胞系 KR-12与 PBMC的融合率 The lack of an ideal parental cell line is one of the important factors hindering the development of human monoclonal antibody lymphocyte hybridoma technology. The authors used peripheral blood mononuclear cells (PBMCs) from patients with hemorrhagic fever with renal syndrome as the source of a unified B lymphocyte. The fusion rate of the four currently used fusion parental cell lines and the hybridoma cells secreted by them are capable of secreting human IgM IgG ratio were compared. The fusion rates of human lymphoblastoid line HF2, mouse myeloma cell line Ag8.653, human-mouse myeloma cell line SHM-D33 and hybrid myeloma cell line KR-12 to PBMC were found