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突变体对增加小麦资源的遗传多样性,克隆和解析重要农艺、产量和抗性相关基因具有重要的意义。本研究以高抗赤霉病小麦品种黄方柱(HFZ)及其11个甲磺酸乙酯(EMS)诱导的纯合突变体(F2~F12,Mu4),以及海盐种(HYZ)及其12个EMS纯合突变体(Y2~Y13,Mu4)为材料,在扬花期利用单花滴注鉴定赤霉病扩展抗性,灌浆期测定了分蘖数、株高、旗叶长、旗叶宽、叶绿素含量,收获后测定千粒重、粒长、粒宽、每穗小穗数和每穗粒数共计11个性状。每一个突变体至少在一个性状上与野生型存在显著差异。黄方柱突变系F6、F9和F12以及海盐种突变系Y6、Y7和Y9病小穗率均显著高于相应野生型,达中感或高感水平,因此,这6个突变体是研究赤霉病扩展抗性的理想材料。此外,海盐种突变体株高、千粒重、每穗粒数和粒宽均低于或显著低于野生型。对野生型和突变体采用了基于最小组内平方和距离的动态聚类分析方法,综合评价了野生型与突变体以及突变体相互之间的相似情况。株高等农艺性状显著优于野生型但赤霉病抗性与野生型类似的突变体(如F2、F7、Y2、Y3、Y4、Y8、Y10和Y12)可为农艺性状的遗传研究和抗赤育种亲本选配提供重要的资源。
Mutants are important for increasing the genetic diversity of wheat resources and for cloning and analyzing important agronomic, yield and resistance-related genes. In this study, homozygous mutants (F2 ~ F12, Mu4) induced by HFZ and eleven ethyl methanesulfonate (EMS), as well as HYZ and 12 EMS homozygous mutants (Y2 ~ Y13, Mu4) were used as material to identify the expansion resistance of Fusarium head blight at flowering stage. The tiller number, plant height, flag leaf length, flag leaf width, chlorophyll Content, grain weight after harvest, grain length, grain width, number of spikelets per panicle and grains per spike a total of 11 traits. Each mutant differs significantly from the wild type in at least one trait. The spikelet rates of F6, F9 and F12 and the Y6, Y7 and Y9 disease lines of Huangfangzhu mutant lines were significantly higher than those of the corresponding wild type, midgame or high susceptible lines. Therefore, The ideal material to extend resistance. In addition, the sea salt mutant plant height, 1000-grain weight, grains per spike and grain width were lower or significantly lower than the wild type. Dynamic clustering analysis based on the least squares squared distance and distance between the wild type and the mutant was used to evaluate the similarity between the wild type and the mutant and the mutant. Mutants such as F2, F7, Y2, Y3, Y4, Y8, Y10 and Y12 with significantly higher agronomic traits than wild type but with scab resistance were similar to those of the wild type Breeding parental matching provides important resources.