用沙门氏菌回复突变试验检出环境中致突变物和致癌物的Ames法正在全世界广泛应用。但是Ames法的测定时间过长,操作手续也比较麻烦。1982年I.Karube等曾报道一种沙门氏菌微生物电极法来检出致突变物,可缩短试验时间,而且比Ames法更灵敏。但此法未考虑试验菌株的自发回变问 The Ames method of detecting mutagenic and carcinogenic agents in the environment by the Salmonella mutation test is widely used all over the world. However, Ames method of measurement time is too long, the operating procedures are too much trouble. In 1982 I. Karube et al. Reported a Salmonella microbial electrode method to detect mutagens, can shorten the test time, and more sensitive than the Ames method. However, this method does not consider the spontaneous return of test strains asked