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目的 研究生长抑素类似物奥曲肽 (OCT)和三苯氧胺 (TAM)联合抗乳腺癌的作用。方法 在体外培养条件下 ,分别或联合用OCT、TAM作用于雌激素受体 (ER)阳性 (MCF 7)和阴性(MDA MB 435S)人乳腺癌细胞株 ,应用MTT比色法分析细胞生长抑制作用 ,流式细胞术测定细胞周期分布和凋亡率 ,透射电镜观察细胞超微结构。 结果 OCT和TAM均可抑制MCF 7细胞生长、阻滞细胞周期于G0 /G1期 ,并可诱导细胞凋亡 ;当两药联合应用时 ,上述作用得到显著加强 ,凋亡率明显上升。OCT和TAM对MDA MB 435S细胞也有弱的抑制作用 ,并阻滞细胞周期于不同时相 ,而未发现明显的凋亡诱导 ,但两药联合应用 ,可诱导 2 2 7%的细胞凋亡。 结论 OCT和TAM联合应用对ER阳性和ER阴性乳腺癌细胞均具有协同抑制生长和诱导凋亡作用 ,可能提高乳腺癌的临床疗效
Objective To study the antitumor effect of the somatostatin analogue octreotide (OCT) and tamoxifen (TAM). Methods OCT and TAM were applied to human breast cancer cell lines with ER (positive) (MCF 7) and negative (MDA MB 435S), respectively, in vitro and in vitro. MTT assay was used to analyze cell growth inhibition. The role of cell cycle distribution and apoptosis rate were determined by flow cytometry. The ultrastructure of the cells was observed by transmission electron microscopy. Results Both OCT and TAM could inhibit the growth of MCF 7 cells, arrest the cell cycle in G0/G1 phase, and induce apoptosis. When the two drugs were combined, the above effects were significantly enhanced and the apoptosis rate increased significantly. OCT and TAM also had a weak inhibitory effect on MDA MB 435S cells and blocked the cell cycle at different phases. No obvious apoptosis induction was found, but the combination of the two drugs could induce apoptosis of 227% of cells. Conclusion The combined use of OCT and TAM has synergistic inhibitory growth and apoptosis-inducing effect on ER-positive and ER-negative breast cancer cells, which may improve the clinical efficacy of breast cancer.