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目的研究应用α干扰素 （Ｉｎｔｅｒｆｅｒｏｎ－ α ， ＩＦＮ－ α）对根治性切除术后肝癌转移复发的抑制作用，以及联合应用全反式维甲酸（ａｌｌ ｔｒａｎｓ ｒｅｔｉｎｏｉｃ ａｃｉｄ， ＡＴＲＡ）是否与ＩＦＮ－α的疗效有协同作用。方法在体外检测ＩＦＮ－α和（或）ＡＴＲＡ对肝癌细胞株ＳＭＭＣ７７２１、ＢＥＬ－７４０２、ＢＥＬ－７４０５、ＭＨＣＣ９７增殖的影响。４４只裸鼠肝内原位接种裸鼠人肝癌转移模型ＬＣＩ－Ｄ２０肿瘤组织，于种植后第１０天切除移植瘤，共分５组，分别为ＩＦＮ－α治疗组（皮下注射， ３×１０５ Ｕ·只一１· ｄ－１） ８只裸鼠， ＩＦＮ－ａ治疗组（６ × １０５ Ｕ·只一１· ｄ－１） ８只裸鼠， ＡＴＲＡ治疗组（灌胃， ２０ ｍｍ· ｋｇ－１· ｄ－１） ８只裸鼠， ＩＦＮ－α联合 ＡＴＲＡ治疗组（ ２０ ｍｍ· ｋｇ－１· ｄ－１灌胃， ＩＦＮ－α ６×１０５ Ｕ·只－１· ｄ－１皮下注射） ８只裸鼠，对照组 １２只裸鼠。于切除后第 ２天开始用药，治疗 ３５ ｄ后处死动物，测量复发瘤大小，观察肝内播散、肝外转移情况。结果 体外实验表明ＩＦＮ－α和ＡＴＲＡ抑制肝癌细胞的增殖作用不明显。对照组裸鼠肺转移率为 １００％（１２／１２）、肝内复?
Objective To study the inhibitory effect of interferon-α (IFN-α) on the metastasis and recurrence of hepatocellular carcinoma after radical resection and whether the combination of all trans retinoic acid (ATRA) and IFN-α The effect is synergistic. Methods The effects of IFN-α and/or ATRA on the proliferation of hepatoma cell lines SMMC7721, BEL-7402, BEL-7405 and MHCC97 were detected in vitro. Forty-four nude mice were orthotopically inoculated in the nude mouse liver cancer metastasis model LCI-D20 tumor tissue. The transplanted tumors were resected on the 10th day after implantation. They were divided into 5 groups: IFN-α treatment group (subcutaneous injection, 3×105, respectively). U. Only one 1.d-1) Eight nude mice, 8 nude mice in the IFN-a treatment group (6 × 105 U. Only one. 1 d-1), ATRA treatment group (gastric administration, 20 mm kg -1· d-1) Eight nude mice, IFN-α combined with ATRA treatment (20 mm·kg-1·d-1 gavage, IFN-α 6×105 U·only -1· d-1 subcutaneous injection 8 nude mice and 12 nude mice in the control group. The drug was administered on the second day after resection and the animals were sacrificed after 35 days of treatment. The size of the recurrent tumor was measured and intrahepatic dissemination and extrahepatic metastasis were observed. Results In vitro experiments showed that IFN-α and ATRA inhibit the proliferation of hepatoma cells is not obvious. The lung metastasis rate in the control group was 100% (12/12), and the liver was recovered.