植物对同种昆虫重复取食做出更有效的响应是植物适应自然界虫害周期性爆发的重要策略。为了研究蒙古沙冬青对其主要害虫灰斑古毒蛾幼虫重复取食响应的代谢基础,本实验采用基于核磁共振(NMR)的代谢组学技术,分析了遭受同一年不同代灰斑古毒蛾幼虫取食的蒙古沙冬青叶片和对照组幼苗叶片的代谢差异。结果表明,仅遭受第一代幼虫取食叶片在第二代幼虫期与对照之间代谢物存在较小差异,而仅遭受第二代幼虫取食叶片与遭受两代幼虫取食叶片代谢差异显著,仅遭受第一代与仅遭受第二代幼虫取食的植株相邻分枝叶片差异显著。另外,只有仅遭受第二代幼虫取食组幼苗表现出处理叶片与相邻分枝叶片的显著代谢差异,并且这种代谢差异为总体代谢的变化,而并未表现为特定代谢物含量变化。由此可见,蒙古沙冬青能在代谢水平以很小的代谢变化来保存昆虫取食信息,在遭受同种昆虫取食后,代谢差异被放大,而且这种信息保存在遭受取食叶片中表现更明显。 Plants respond more efficiently to repetitive feeding of the same species of plants as an important strategy for plants to adapt to the cyclical outbreaks in nature. In order to study the metabolic basis of A. mongolica on the repeated feeding responses of A. mongolica to its major insect pest, the study was conducted on the basis of nuclear magnetic resonance (NMR) -based metabonomics techniques, Metabolic differences in the leaves of edible Mongolian sandvine leaves and the control seedlings. The results showed that only the first-generation larvae fed on leaves had minor differences in metabolites between the second larvae and the control, but only the second-generation larvae fed on the leaves and the larvae fed on the larvae of the second generation showed significant differences in metabolism , Only the first generation and the second-generation larvae only suffer from the adjacent branches and leaves of plants significantly different. In addition, only the second-generation larvae fed the seedlings showed significant metabolic differences between the treated leaves and the adjacent branched leaves, and this metabolic difference was an overall metabolic change without showing any change in the specific metabolite content. Thus, the Mongolian sand holothuric can be stored at a small metabolic level of metabolic changes in insect feeding information, after being subjected to the same species of insects feeding, the metabolic differences are amplified, and this information is stored in the performance of feeding leaves More obvious.