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目的：分离编码人嗜酸性细胞趋化素（ｈＥｏｔ）的基因ｃＤＮＡ，并将它克隆至质粒ｐＵＣ１８中进行核苷酸序列分析。方法：采用ＴＮＦα刺激人肺上皮细胞提取总ＲＮＡ，利用ＲＴ－ＰＣＲ方法获得了ｈＥｏｔ的ｃＤＮＡ，并将其定向插入ｐＵＣ１８载体中进行核苷酸序列。结果：ＤＮＡ序列分析表明分离的ｈＥｏｔｃＤＮＡ的序列与文献报道的完全一致。结论：本研究获得了序列正确的ｈＥｏｔ基因ｃＤＮＡ，为下一步的研究打下基础。
OBJECTIVE: To isolate the cDNA encoding human eotaxin (hEot) and cloned into plasmid pUC18 for nucleotide sequence analysis. Methods: Human lung epithelial cells were stimulated with TNFα for total RNA extraction. CDNA of hEot was obtained by RT-PCR and inserted into pUC18 vector for nucleotide sequence. Results: DNA sequence analysis showed that the sequence of isolated hEot cDNA was completely consistent with that reported in the literature. Conclusion: The hEot gene cDNA with the correct sequence was obtained in this study, which laid the foundation for further study.