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现将1983年在日本农林水产省蔬菜试验场进修期间应用过的几种番茄抗病性鉴定方法,整理如下,供有关育种工作者参考。一、烟草花叶病毒(简称TMV) (一)毒源的准备与保存:毒源可使用冷冻干燥之毒叶,也可使用新鲜毒叶。 1.接毒液的调制:使用冷冻干燥毒叶时,将干叶放入研体内,加入少量1/15M的磷酸缓冲液(1/15M的K_2HPO_4与1/15M的KH_2PO_4,比列为6:4)。磨碎后,再用
Now in 1983 in Japan’s agriculture, forestry and fisheries provinces vegetable test field during the training of several tomato disease resistance identification methods, organized as follows, for reference breeders. First, the tobacco mosaic virus (referred to as TMV) (A) preparation and preservation of poison sources: poison source freeze-dried leaves can also be used, but also the use of fresh poisonous leaves. 1. Preparation of toxins: When freeze-dried leaflets are used, the dried leaves are placed in the medium and a small amount of 1 / 15M phosphate buffer (1/15 M K 2 HPO 4 and 1/15 M KH 2 PO 4, 6: 4 ). After grinding, reuse