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目的改进和完善大鼠再灌注心律失常在体模型的制作方法。方法将36只雄性Sprague-Dawley大鼠随机分为假手术组(n=8)、模型组(n=20)、胺碘酮组(n=8,3mg·kg~(-1),舌下静脉注射)。麻醉动物后,钝性分离大鼠3、4肋间肌,用自制小拉钩拉开胸腔,“双线法”结扎左冠状动脉前降支,经8min缺血后剪断结扎线,再灌注30min,观察各组死亡率,实验中用PowerLab连续描记心电图,分析再灌注期心电图变化。结果模型组再灌注期100%出现明显室性心律失常,包括心室纤颤、室性心动过速、室性早搏等,恶性心律失常引起的死亡率为55%;胺碘酮可有效控制心律失常,仅发生室性早搏,死亡率为0(P<0.01)。结论改良方法制备的大鼠再灌注心律失常的动物模型的重复性好,成功率高。
Objective To improve and perfect the method of making rat model of reperfusion arrhythmia in vivo. Methods Thirty - six male Sprague - Dawley rats were randomly divided into sham operation group (n = 8), model group (n = 20), amiodarone group (n = 8 and 3 mg · kg ~ Intravenous injection). After the animals were anesthetized, the intercostal muscles of rats 3 and 4 were blunt dissected, and the thoracic cavity was opened with self-made small pull hooks. The anterior descending branch of left coronary artery was ligated by “double-line method” 30min, observed the mortality of each group, the experiment with PowerLab continuous electrocardiogram, analysis of electrocardiogram changes during reperfusion. Results In model group, obvious ventricular arrhythmia occurred in 100% of reperfusion period, including ventricular fibrillation, ventricular tachycardia and ventricular premature beat. The mortality rate of malignant arrhythmia was 55%. Amiodarone could effectively control arrhythmia , Only premature ventricular contractions, the mortality rate was 0 (P <0.01). Conclusion The animal model of reperfusion arrhythmia prepared by the improved method has good repeatability and high success rate.