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目的研究臭氧暴露对支气管哮喘大鼠肺功能及气道炎症的影响。方法采用随机数表法,将24只雄性SD大鼠分为3组,每组8只,分别为正常对照组、模型对照组、臭氧暴露组。模型对照组采用卵蛋白(OVA)腹腔注射致敏,结合OVA持续雾化吸入复制经典哮喘模型;臭氧暴露组在支气管哮喘模型的基础上叠加臭氧暴露。观察记录各组大鼠一般情况,肺功能,支气管肺泡灌洗液(BALF)行瑞氏染色,显微镜下进行炎症细胞分类计数。结果肺功能检测显示与正常对照组相比,模型对照组和臭氧暴露组中心气道阻力(Rn)及气道高反应性(I)升高,差异有统计学意义(P<0.05),提示两组均有气流受限及换气功能障碍;模型对照组BALF中炎性细胞总数高于正常对照组,臭氧暴露组高于模型对照组,差异有统计学意义(P<0.05)。结论臭氧暴露导致支气管哮喘大鼠肺功能下降,同时导致支气管肺泡灌洗液中炎性细胞增多。
Objective To study the effects of ozone exposure on lung function and airway inflammation in bronchial asthmatic rats. Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups (n = 8 each), which were normal control group, model control group and ozone exposure group. The model control group was sensitized by intraperitoneal injection of ovalbumin (OVA), followed by repeated inhalation of OVA to replicate the classical asthma model. Ozone exposure group was overexposed to ozone on the basis of bronchial asthma model. The general conditions of rats in each group were observed and recorded. The lung function and bronchoalveolar lavage fluid (BALF) were analyzed by Wright staining. The number of inflammatory cells was counted by microscope. Results Pulmonary function tests showed that the airway resistance (Rn) and airway hyperresponsiveness (I) in the model control group and the ozone exposure group were significantly higher than those in the normal control group (P <0.05), suggesting The airflow limitation and ventilatory dysfunction were found in both groups. The total number of inflammatory cells in BALF of model control group was higher than that of normal control group, and the ozone exposure group was higher than that of model control group (P <0.05). Conclusion Ozone exposure leads to decreased lung function in bronchial asthmatic rats and increased inflammatory cells in bronchoalveolar lavage fluid.