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目的研究大黄素(emodin)对蛋氨酸-胆碱缺乏(MCD)饲料诱导的小鼠非酒精性脂肪性肝病(NAFLD)的影响。方法C57BL/6J小鼠随机分为3组,分别为添加蛋氨酸-胆碱饲料组(MCS)、MCD饲料联合DMSO注射组(MCD)、MCD饲料联合emodin注射组(MCD-emodin)。三组小鼠喂养相应饲料,10 d后,每天腹腔分别注射DMSO或emodin,连续注射20 d。HE染色观察肝脏病理改变;全自动生化分析仪检测血清谷氨酸氨基转移酶(ATL)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、碱性磷酸酶(ALP)和葡萄糖(GLU)水平;实时定量PCR技术检测肝脏白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)的水平。结果 HE染色显示,与MCS组相比,MCD饲料组引起明显的脂肪变性和炎性细胞浸润,且注射emodin后脂肪变性及炎性细胞浸润明显增加;与单纯MCD饲料组相比,emodin注射后,血清ALT和AST水平显著升高,IL-1β和IL-6的mRNA水平明显上升。结论 Emodin加重MCD饲料诱导的NAFLD。
Objective To investigate the effect of emodin on mice with non-alcoholic fatty liver disease (NAFLD) induced by methionine-choline deficiency (MCD). Methods C57BL / 6J mice were randomly divided into 3 groups: MCF group, MCD group and MCD group (MCD-emodin). The mice in the three groups were fed the corresponding diets. After 10 days, DMSO or emodin was injected intraperitoneally every day for 20 days. The pathological changes of the liver were observed by HE staining. The contents of serum glutamate aminotransferase (ATL), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC) (HDL), low density lipoprotein (LDL), alkaline phosphatase (ALP) and glucose (GLU) were detected by real-time quantitative PCR. The levels of interleukin 1β, interleukin 6 -6), tumor necrosis factor alpha (TNF-alpha) levels. Results HE staining showed that compared with MCS group, the MCD diet group caused obvious steatosis and inflammatory cell infiltration, and the fatty degeneration and infiltration of inflammatory cells were significantly increased after injection of emodin. Compared with the MCD group, , Serum ALT and AST levels were significantly increased, IL-1β and IL-6 mRNA levels increased significantly. Conclusion Emodin aggravates MCD-induced NAFLD.