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目的:研究生半夏及干姜人参半夏丸的一般毒性和胚胎毒性。方法:采用一般毒性实验方法及流式细胞术比较了两者对未孕小鼠体质量、脏器及T淋巴细胞亚群的影响;采用致畸敏感期生殖毒性方法比较了两者对孕鼠妊娠及胚胎发育的影响。结果:连续给药14d后,生半夏粉组小鼠体质量增重显著降低(P<0.05)、肾脏毒性明显(P<0.05);致畸敏感期给药10d后,致孕鼠心、肾病理改变明显,妊娠18d(GD18)体质量、宫外增重及胎仔身长显著低于正常组。而干姜人参半夏粉高、低剂量对孕鼠及胎仔均无明显的毒性,但同生半夏粉一样引起小鼠脾脏CD4+T明显降低而CD8+T显著升高(P<0.05)。结论:生半夏粉与干姜、人参配伍后对孕体及胎儿没有显著毒性,但仍可能有一定的免疫抑制作用,其原因与作用机制值得进一步研究。
Objective: To study the general toxicity and embryotoxicity of Pinellia ternata and Ginger Ginseng Pinellia Pills. Methods: The effects of both on the weight, organs and T lymphocyte subsets in nonpregnant mice were compared by general toxicity test and flow cytometry. The reproductive toxicity of teratogenicity was compared between pregnant rats The impact of pregnancy and embryonic development. Results: The weight gain of rats in the raw Pinellia ternata powder group was significantly decreased (P <0.05) and the renal toxicity was significant (P <0.05) after 14 days of continuous administration; Pathological changes of kidney were obvious. Body weight, extra-uterine weight gain and fetal size in GD18 were significantly lower than those in normal group at 18d. However, Ginger and Ginseng Pinellia powder had no significant cytotoxicity on pregnant rats and fetuses, but the same as the control group, the spleen CD4 + T and CD8 + T were significantly increased (P <0.05) . Conclusion: There is no significant toxicity on the pregnant body and fetus when the raw pinellia powder is mixed with ginger and ginseng, but it may still have some immunosuppressive effects. The reason and mechanism of the combination are worth further study.