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目的研究输血相关急性肺损伤(TRALI)大鼠肺组织细胞因子诱导的中性粒细胞趋化因子1(CINC-1)mRNA表达水平的变化,探讨CINC-1在大鼠TRALI发病机制中的作用。方法将60只SD大鼠随机分为TRALI组、正常对照组、脂多糖(LPS)对照组和阳性对照组,每组15只大鼠。LPS对照组大鼠经腹腔注射LPS(2 mg/kg,1 min内完毕),2 h后静脉输注1 m L生理盐水;TRALI组大鼠经腹腔注射LPS(2 mg/kg)2 h后静脉输注1 m L储存28 d的同种异体血浆;正常对照组大鼠采用假手术处理;阳性对照组大鼠移除约等于大鼠10%血容量的血液(1 m L)后,经静脉输注LPS(5 mg/kg)诱导急性肺损伤。采用逆转录聚合酶链反应检测大鼠肺组织中CINC-1 mRNA表达水平,采用酶联免疫吸附试验测定肺组织匀浆CINC-1蛋白含量;检测肺湿/干重比、肺组织髓过氧化物酶(MPO)活性,观察肺组织病理学改变,比较支气管肺泡灌洗液(BALF)中细胞总数和中性粒细胞百分比。结果与正常对照组和LPS对照组比较,TRALI组肺组织中CINC-1蛋白和CINC-1 mRNA表达明显增高[(18.98±2.21)ng/g和[(26.42±3.37)ng/g比(38.95±4.32)ng/g,F=11.28,P<0.05;(0.24±0.09)和(0.15±0.08)比(0.38±0.09),F=15.31,P<0.05]。TRALI组BALF中细胞总数和中性粒细胞百分比[(310.63±76.67)×106/L和(33.57±11.51)%]明显高于正常对照组[(101.36±63.83)×106/L和(9.87±3.56)%](F=13.82、5.41,P<0.05)。TRALI组肺组织含水量和MPO活性明显高于正常对照组(F=5.78、13.46,P<0.05)。阳性对照组各指标均显著高于TRALI组(P<0.05)。结论 TRALI大鼠肺组织中CINC-1的表达升高,肺组织中性粒细胞浸润增多,提示CINC-1参与了中性粒细胞与内皮细胞的粘附和聚集,推测CINC-1可能在TRALI的发病过程中起着重要作用。
Objective To investigate the changes of cytokine-induced neutrophil chemotactic factor 1 (CINC-1) mRNA expression in lungs of rats with transfusion-associated acute lung injury (TRALI) and to explore the role of CINC-1 in the pathogenesis of TRALI in rats . Methods Sixty SD rats were randomly divided into TRALI group, normal control group, lipopolysaccharide (LPS) control group and positive control group, 15 rats in each group. Rats in LPS control group were injected intraperitoneally with LPS (2 mg / kg for 1 min), and 2 ml of normal saline was infused 2 hours later. Rats in TRALI group were intraperitoneally injected with LPS (2 mg / kg) for 2 h The rats in the normal control group were treated with sham operation by intravenous infusion of 1 m L of allogeneic plasma for 28 days. After the rats in the positive control group had removed the blood (1 mL) which was about 10% of the blood volume in rats, Intravenous infusion of LPS (5 mg / kg) induces acute lung injury. The expression of CINC-1 mRNA in lung tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR). The content of CINC-1 protein in lung homogenate was determined by enzyme-linked immunosorbent assay (ELISA) (MPO) activity were observed in lung tissue pathological changes in bronchoalveolar lavage fluid (BALF) in the total number of cells and neutrophils percentage. Results Compared with normal control group and LPS control group, the expression of CINC-1 protein and CINC-1 mRNA in lung tissue of TRALI group was significantly higher than that of normal control group [(18.98 ± 2.21) ng / g and [(26.42 ± 3.37) ng / g ± 4.32) ng / g, F = 11.28, P <0.05; (0.24 ± 0.09) and (0.15 ± 0.08) vs (0.38 ± 0.09), F = 15.31, P <0.05]. The total number of cells and the percentage of neutrophils in BALF in TRALI group were significantly higher than those in normal control group [(310.63 ± 76.67) × 106 / L and (33.57 ± 11.51)%] [(101.36 ± 63.83) × 106 / L and 3.56)%] (F = 13.82, 5.41, P <0.05). The lung tissue water content and MPO activity in TRALI group were significantly higher than those in normal control group (F = 5.78, 13.46, P <0.05). The positive control group indicators were significantly higher than the TRALI group (P <0.05). Conclusions The expression of CINC-1 in lung tissue of TRALI rats is increased, and the infiltration of neutrophils in lung tissue is increased, suggesting that CINC-1 is involved in the adhesion and aggregation of neutrophils to endothelial cells. It is speculated that CINC-1 may be involved in TRALI The pathogenesis plays an important role.