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目的:探讨急性低压缺氧后内皮细胞的超微结构变化及其机理。方法:将20只成熟健康Wis-tar大鼠分成两组,每组10只,将缺氧组大鼠放入低压舱内,以15m/s速度上升至7500m高度,停留30分钟,造成体内重度缺氧。对照组实验条件相同,但不作上升。结果:缺氧后实验组动物心肌组织中内皮素(ET)含量明显下降;硒谷胱甘肽过氧化物酶(SeGSHpx)浓度显著减少;丙二醛(MDA)改变不明显;SeGSHpx/MDA比值显著降低;心肌线粒体钙含量明显增加;心肌终末微血管内皮细胞超微结构发生明显改变。结论:低压缺氧后内皮细胞超微结构改变可能与ET浓度的变化、组织细胞钙超载及抗氧化能力下降有关。
Objective: To investigate the ultrastructural changes of endothelial cells after acute hypobaric hypoxia and its mechanism. Methods: Twenty healthy and healthy Wis-tar rats were divided into two groups, 10 rats in each group. The rats in hypoxia group were placed in the low-pressure cabin and were raised to a height of 7500m at a speed of 15m / s for 30 minutes. Hypoxia. The control group had the same experimental conditions but no increase. Results: After hypoxia, the content of endothelin (ET) in myocardium of the experimental group decreased significantly; the concentration of SeGSHpx decreased significantly; but the change of malondialdehyde (MDA) was insignificant; the ratio of SeGSHpx / MDA Significantly decreased; myocardial mitochondrial calcium content increased significantly; myocardial terminal microvascular endothelial cell ultrastructure was significantly changed. CONCLUSION: The ultrastructural changes of endothelial cells after hypobaric hypoxia may be related to the change of ET concentration, the overloading of calcium and the decrease of antioxidant capacity.