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目的掌握拉萨市蚊虫种类构成及密度动态,为该市媒介蚊虫及蚊媒传染病监测及防控提供基础数据。方法在拉萨市城区不同方位选取调查点,以诱蚊灯法、人帐诱法及人工小时法捕获蚊类,用形态学和多重PCR方法进行蚊种鉴定。结果拉萨市蚊类包括尖音库蚊复合组蚊虫亚种和骚扰阿蚊,优势蚊种为尖音库蚊复合组蚊虫亚种。2009、2012年人帐诱法总蚊密度分别为19.57和3.06只(/帐·h),不同年份不同调查点蚊密度差异无统计学意义(F1,1=0.934,P>0.05)。2009、2012年人工小时法总蚊密度分别为62.10和7.90只/人工小时,不同年份不同调查点蚊密度差异无统计学意义(F1,1=0.874,P>0.05)。2012、2013年诱蚊灯法总蚊密度分别为1.47和0.20只(/灯·h)。不同年份不同调查点居民区蚊密度差异亦无统计学意义(F1,3=1.503,P>0.05)。2012年诱蚊灯法捕获的105只蚊虫中,经多重PCR鉴定,36只属尖音库蚊复合组蚊虫亚种纯合子(34.29%),69份出现杂交(65.71%)。结论拉萨市存在较稳定尖音库蚊复合组蚊虫,蚊密度相对较低,提示该地区存在蚊虫及蚊媒传染病风险,应对该地区蚊虫及蚊媒传染病开展监测与风险评估,为蚊虫及蚊媒传染病控制提供决策依据。
Objective To master the species composition and density dynamics of mosquitoes in Lhasa and provide the basic data for the monitoring and control of mosquito vector and mosquito vector in the city. Methods The survey sites were selected from different directions in the urban area of Lhasa. Mosquitoes were captured by the mosquito lamp method, the human-led accounting method and the artificial hour method. The mosquito species were identified by morphological and multiplex PCR methods. Results The mosquitoes in Lhasa City included mosquito Culex pipiens complex and mosquitoes mosquitoes, and the dominant mosquito species were mosquito Culex. In 2009 and 2012, the total mosquito densities of human-animal accounts were 19.57 and 3.06, respectively. There was no significant difference in mosquito density between different surveys in different years (F1,1 = 0.934, P> 0.05). The total mosquito density of artificial insemination in 2009 and 2012 were 62.10 and 7.90 / artificial hours, respectively. There was no significant difference in mosquito density among different years (F1,1 = 0.874, P> 0.05). The total mosquito density of the trapping lamp method was 1.47 and 0.20 (/ lamp · h) in 2012 and 2013, respectively. There was no significant difference in mosquito density among residential areas at different survey sites in different years (F1,3 = 1.503, P> 0.05). Of the 105 mosquitoes captured by the mosquito lamp method in 2012, 36 were homozygous mosquitoes (34.29%) and 69 (65.71%) were identified by multiplex PCR. Conclusion There is a relatively low density of mosquitoes in Culex pipiens complex group in Lhasa, which indicates the mosquito and mosquito-borne infectious disease in this area. Monitoring and risk assessment of mosquito and mosquito-borne infectious diseases should be carried out in this area. Mosquito-borne disease control provides the basis for decision-making.