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斑茅(Erianthus arundinaceus)是甘蔗育种的重要种质资源,同时也是潜在的能源草植物,对其进行遗传多样性研究是合理利用该资源的前提。本研究利用相关序列扩增多态性(SRAP)分子标记对我国7个省的45份野生斑茅种质资源进行遗传多样性研究,结果表明,1)20对引物组合共扩增出434条清晰的条带,多态性条带300条,占69%,材料间的遗传相似性系数(GS)范围为0.703~0.986,平均GS为0.842,说明我国野生斑茅资源具有丰富的遗传多样性;2)通过非加权配对类平均法(UPGMA)聚类分析和主成分分析发现,供试材料可以分为3个大类7个亚类,聚类结果呈现出较强的地域分布规律,材料间的遗传距离与地理距离有一定的相关性;3)我国野生斑茅资源的遗传多样性及聚类结果受地理条件的影响,大洋、山体能阻碍不同地域斑茅间的基因交流,而河流能促进基因交流。本研究揭示了我国野生斑茅的遗传多样性水平,阐明了影响其遗传多样性的主要因素,为斑茅的开发利用及甘蔗的遗传改良育种提供重要基础资料。
Erianthus arundinaceus is an important germplasm resource for sugarcane breeding and a potential plant of energy grass, and its genetic diversity is a prerequisite for rational utilization of this resource. In this study, we studied the genetic diversity of 45 accessions from wild germplasm resources in seven provinces of China using SRAP molecular markers. The results showed that 1) 20 pairs of primer combinations amplified 434 There were 300 bands (69%) with clear bands and polymorphic bands, and the genetic similarity coefficient (GS) ranged from 0.703 to 0.986 and the average GS was 0.842, which indicated that the wild Zea resources in our country is rich in genetic diversity ; 2) By means of UPGMA cluster analysis and principal component analysis, we found that the tested materials can be divided into 7 sub-categories of 3 major categories and the clustering results show a strong geographical distribution. The materials The genetic distance between them and the geographical distance have a certain correlation; 3) The genetic diversity and clustering results of the wild echinacea in China are influenced by the geographical conditions, and the oceans and mountains can hinder the gene exchange between the two regions; Can promote gene exchange. This study revealed the genetic diversity of wild M.grass in China, clarified the main factors that affect its genetic diversity, and provided important basic data for the development and utilization of M.grasses and the genetic improvement breeding of sugarcane.