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1999年 2～ 5月 ,东山县养殖九孔鲍暴发了大规模流行病 ,不少养殖场全场覆没 .病鲍表现为分泌粘液增多、肝脏红肿、足部僵硬和反应迟钝 .应用磷钨酸负染、超薄切片的电镜观察和现场检测等方法 ,对病鲍的病原及肝肠组织的病理情况进行观测 ,结果表明引发这次养殖鲍暴发严重病害的主要病原是致病力很强的病毒和弧菌 .电镜观察到病毒发生在细胞质中的一种称为“封入体”的泡状结构中 ,证实了病原的入侵造成九孔鲍肝及肠等组织、细胞产生病变 ,描述了细菌和病毒混合感染导致九孔鲍细胞的病理变化
From February to May 1999, a large-scale epidemic disease was reported in the breed of Abalone in Dongshan County, and many farms were overwhelmed with diseases such as increased secretion of mucus, swelling of the liver, stiff and unresponsive foots.Application of phosphotungstic acid Negative staining, ultra-thin sections of the electron microscopy and field testing and other methods to diseased Bao pathogen and the pathological conditions of the liver and intestine were observed, the results show that the main cause of the outbreak of serious disease Baoying Bao is a highly virulent virus And Vibrio.Electron microscopy showed that the virus occurred in the cytoplasm of a so-called “envelope” of the vesicular structure, confirmed that the invasion of pathogens caused by nine holes abalone and intestinal and other tissues, cells produce lesions, described the bacteria and Mixed infection of the virus causes pathological changes in nine-hole abalone cells