通过表达序列标志（ＥＳＴ）测序、ＳＡＧＥ分析和ｃＤＮＡ杂交等方法，人们已构建了十分详细的基因转录图谱，获得了有关人类基因组编码序列及表达时空的资料，在人类肿瘤致病基因的克隆、鉴定、功能分析以及全面了解肿瘤发展过程的分子基础发挥了重要作用。大量ＥＳＴ的获得为定位候选克隆法提供了候选基因的标志，为克隆到的基因片段提供了相关资料，并且成为把模式生物基因组的研究成果运用到人类肿瘤研究的桥梁；通过构建肿瘤基因表达差异图谱，人们可以全面了解肿瘤发生发展过程中的基因表达改变 By means of EST sequencing, SAGE analysis and cDNA hybridization, a very detailed gene transcription profile has been constructed and data about the human genome coding sequence and expression space have been obtained. In the cloning, Identification, functional analysis and a comprehensive understanding of the molecular basis of the tumor development process has played an important role. Obtaining a large number of ESTs provides candidate gene markers for positioning candidate cloning methods, providing relevant information for the cloned gene fragments and becoming a bridge for applying the research results of the model biological genomes to human tumor research. By constructing tumor gene expression differences Atlas, one can fully understand the gene expression changes during tumorigenesis