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目的:研究5-烯丙基-7-二氟甲基白杨素(ADFMChR)诱导人急性髓性白血病HL-60细胞分化作用。方法:体外培养人急性髓性白血病HL-60细胞。瑞氏-吉姆萨染色法观察细胞分化的形态学改变;细胞免疫组织化学法和间接免疫荧光显微镜观察细胞表面分化抗原CD11b表达。结果:瑞氏-吉姆萨染色结果显示,ADFMChR诱导HL-60细胞发生分化成熟的形态学变化;细胞免疫组织化学显示ADFMChR增加HL-60细胞粒细胞系的特异性表面分化抗原CD11b的表达;间接免疫荧光显示经ADFMChR处理的HL-60细胞表面分化抗原CD11b表达增强,阳性表达率呈剂量依赖性升高。结论:ADFMChR具有诱导HL-60细胞分化的作用。
AIM: To investigate the differentiation of human acute myeloid leukemia HL-60 cells induced by 5-allyl-7-difluoromethylchrysin (ADFMChR). Methods: Human acute myeloid leukemia HL-60 cells were cultured in vitro. Wright-Giemsa staining to observe the morphological changes of cell differentiation; cell immunohistochemistry and indirect immunofluorescence microscopy of cell surface differentiation antigen CD11b expression. Results: Wright-Giemsa staining showed that ADFMChR induced morphological changes of HL-60 cells. Immunohistochemistry showed that ADFMChR increased the expression of specific surface antigen CD11b in HL-60 cells. Immunofluorescence showed that the expression of surface differentiation antigen CD11b in HL-60 cells treated with ADFMChR increased in a dose-dependent manner. Conclusion: ADFMChR induces the differentiation of HL-60 cells.