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为研究芬太尼对豚鼠单一心室肌细胞 ATP 敏感钾通道(K_(ATP)通道)离子流(I_K_(ATP))的影响,采用膜片钳全细胞方式记录 I_K_(ATP),设保持电位为-40mV,指令电位为-100～30mV,令电极内液含 ATP 0.3mmol·L~(-1)。当保持电位为-40mV,指令电位为0mV 时,加入芬太尼2.4×10~(-3)mmol·L~(-1)后,电流值由给药前的1.053±0.172 nA 减至给药后的0.703±0.114 nA(n=3,P<0.05);加入芬太尼4.8×10~(-3)mmol·L~(-1)后,电流值由给药前的0.990±0.176 nA 减至给药后的0.693±0.021 nA(n=3,P<0.05)。对 I_K_(ATP)的平均抑制率分别为35.0±9.5%(P<0.05)、29.7±16.8%(P<0.05)。其他指令电位下 I_K_(ATP)的改变也符合该趋势。结论:芬太尼能抑制豚鼠单一心室肌细胞 K_(ATP)通道。
To study the effect of fentanyl on ATP-sensitive potassium channel (K_ (ATP)) channel currents in single ventricular myocytes of guinea pigs, patch-clamp whole cell recording of I_K_ (ATP) -40mV, command potential -100 ~ 30mV, so that the electrode solution containing ATP 0.3mmol·L -1. When the holding potential was -40 mV and the command potential was 0 mV, the current value decreased from 1.053 ± 0.172 nA before administration to 2.4 × 10 -3 mmol·L -1 fentanyl administration (N = 3, P <0.05). After adding 4.8 × 10 -3 mmol·L -1 fentanyl, the current value decreased from 0.990 ± 0.176 nA before administration 0.693 ± 0.021 nA after administration (n = 3, P <0.05). The average inhibitory rates of I_K_ (ATP) were 35.0 ± 9.5% (P <0.05) and 29.7 ± 16.8% (P <0.05), respectively. Changes in I_K_ (ATP) for other command potentials also fit this trend. Conclusion: Fentanyl can inhibit K (ATP) channel of single ventricular myocytes in guinea pigs.