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目的 探讨二乙基二硫代氨基甲酸酯 (DDC )对提高纳米级阿霉素碘油乳剂在体内外的抗肿瘤作用。方法 用MTT试验评价纳米级阿霉素碘油乳剂对耐药细胞及肿瘤细胞的杀灭作用 ,用SD大鼠建立Walk -2 5 6肝癌模型 ,通过肝动脉分别注入生理盐水、阿霉素、纳米级阿霉素碘油乳剂 ,DDC加纳米级阿霉素碘油乳剂以及DDC加阿霉素脂质体碘油乳剂 ,分别测定各组的肿瘤生长率和小鼠生命延长率。结果 经过DDC预处理后 ,阿霉素对阿霉素耐药肿瘤细胞SGC 790 1/CVR和SGC 790 /WT的IC5 0 (μg/ml)分别从原来的 18.40降至 0 .74和 4.0 0降至 0 .3 2。未经DDC处理过的各组平均肿瘤生长率远高于预先用DDC处理过的各组 (P <0 .0 1)。而小鼠生命延长率则明显低于预先用DDC处理过的各组 (P <0 .0 5 )。结论 用DDC预先处理抑制肿瘤细胞中的超氧化物歧化酶 (SOD)可提高阿霉素的抗肿瘤作用。
Objective To investigate the anti-tumor effect of diethyldithiocarbamate (DDC) on the enhancement of nanoscale doxorubicin lipiodol emulsion in vivo and in vitro. Methods MTT assay was used to evaluate the killing effect of nanoscale doxorubicin lipiodol emulsion on drug-resistant cells and tumor cells. The model of Walk-256 hepatocellular carcinoma was established with SD rats. Normal saline, doxorubicin, Nano-doxorubicin lipiodol emulsion, DDC plus nanoscale doxorubicin lipiodol emulsion and DDC plus doxorubicin liposome lipiodol emulsion were measured in each group of tumor growth rate and mouse life extension rate. Results After pretreatment with DDC, the IC 50 (μg / ml) of doxorubicin to doxorubicin-resistant tumor cells SGC 790 1 / CVR and SGC 790 / WT decreased from 0.40 to 0.74 and 4.0 0 respectively To 0 .3 2. The mean tumor growth rates for each group without DDC were much higher than those previously treated with DDC (P <0.01). While the mouse life extension rate was significantly lower than the group treated with DDC (P <0.05). Conclusion Pretreatment with DDC inhibits the superoxide dismutase (SOD) in tumor cells can enhance the anti-tumor effect of doxorubicin.