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背景:组织毛细血管增多,可缩短氧从毛细血管向组织细胞弥散的距离,改善组织供氧。但组织毛细血管密度与组织的代谢水平密切相关,单纯缺氧能否引起骨骼肌毛细血管密度增加尚有争议。目的:观察不同缺氧时间大鼠骨骼肌毛细血管密度和血流供应的变化规律。设计:非随机对照实验。对动物缺氧前体质量加以选择,使各组动物的体质量在缺氧末期基本接近。地点和对象:实验地点:第三军医大学病理生理学与高原生理学教研室。清洁级纯种健康Wistar大鼠64只,雌雄各半,分为4组:对照组、缺氧5d组、缺氧15d组和缺氧30d组。干预:缺氧5d组、15d组和30d组大鼠在模拟海拔5000m的减压舱中分别连续减压缺氧5,15和30d后(23h/d),对照组大鼠在舱外饲养。用肌球蛋白ATP酶(mATPase)组织化学方法显示骨骼肌Ⅰ,Ⅱ型纤维和毛细血管并进行图像分析,测定骨骼肌纤维横断面积和毛细血管密度以及单位面积内毛细血管数与肌纤维数的比值;用放射性微球法测定骨骼肌血流量和血管阻力。主要观察指标:①各组大鼠血球压积和骨骼肌血流量、血管阻力的变化。②大鼠腓肠肌Ⅰ,Ⅱ型纤维横断面积。③各组大鼠骨骼肌毛细血管密度和单位面积内毛细血管数与肌纤维数的比值。结果:缺氧5d组大鼠与对照组相比,骨骼肌纤维横断面积即出现显著缩小犤I型分别为(2254±
Background: Tissue capillaries increase, can shorten the oxygen diffusion distance from the capillaries to the tissue cells, improve tissue oxygen supply. However, the tissue capillary density and tissue metabolism are closely related to the simple hypoxia can cause increased skeletal muscle capillary density is still controversial. Objective: To observe the changes of skeletal muscle capillary density and blood flow in rats with different hypoxia time. Design: Non-randomized controlled experiment. The animal hypoxia precursor quality to be selected, so that the body weight of each group of animals in the late hypoxia basically close. Location and Subjects: Experimental Location: Department of Pathophysiology and Altitude Physiology, Third Military Medical University. Sixty-four pure Wistar rats were divided into 4 groups: control group, hypoxia 5d group, hypoxia 15d group and hypoxia 30d group. Intervention: Rats in hypoxia 5d group, 15d group and 30d group were subjected to hypodermic hypoxia for 5, 15 and 30 days (23h / d), respectively. Rats in control group were housed outside of the cage. The myofibrillar ATPase (mATPase) histochemical method was used to display skeletal muscle fibers type Ⅰ and Ⅱ and capillary. Image analysis was performed to determine the skeletal muscle fiber cross - sectional area and capillary density, as well as the ratio of capillary number to muscle fiber number per unit area. Skeletal muscle blood flow and vascular resistance were determined by radioactive microspheres method. MAIN OUTCOME MEASURES: ① Changes of hematocrit and skeletal muscle blood flow and vascular resistance in rats in each group. ② rat gastrocnemius Ⅰ, Ⅱ fiber cross-sectional area. ③ The skeletal muscle capillary density and the ratio of the number of capillaries to the number of myofibers per unit area of rats in each group. Results: Compared with the control group, the cross-sectional area of skeletal muscle of rats in hypoxia 5d group was significantly reduced, the I type were (2254 ±