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为观察儿童支气管哮喘时Ｔ淋巴细胞亚群分布以及Ｔ细胞活化相关因子及受体的表达状况，应用放射免疫分析等技术，对３４例发作期、２５例缓解期支气管哮喘患儿和１５例正常对照的外周血Ｔ淋巴细胞亚群、血浆及淋巴细胞膜表面白细胞介素－２受体（ＩＬ－２Ｒ）和相关细胞因子等水平进行系统检测。结果：（１）发作期患儿外周血Ｔ细胞亚群ＣＤ３，ＣＤ４及ＣＤ４／ＣＤ８值与缓解期患儿及正常对照比较差异无显著意义，但发作期ＣＤ８高于正常对照（Ｐ＜０．０１）和缓解组（Ｐ＜０．０１）；（２）发作期患儿血浆可溶性ＩＬ－２Ｒ、（ｓＩＬ－２Ｒ）、ＩｇＥ水平明显高于缓解期患儿和正常对照（Ｐ＜０．０１）；（３）免疫电镜观察显示，发作期患儿淋巴细胞膜表面ＩＬ－２Ｒ表达高于正常对照（Ｐ＜０．０５）。提示：（１）哮喘患儿外周Ｔ淋巴细胞亚群的分布发生了变化，哮喘发作期Ｔ淋巴细胞处于激活状态；（２）血浆ｓＩＬ－２Ｒ、ＩｇＥ水平与哮喘病情变化密切相关，可作为临床哮喘病情监测的指标。
To observe the distribution of T lymphocyte subsets and the expression of T cell activation-related factors and receptors in children with bronchial asthma, radioimmunoassay and other techniques were used to detect the expression of T lymphocyte subsets in 34 patients with bronchial asthma and 15 normal The levels of peripheral blood T lymphocyte subsets, plasma and lymphocyte surface interleukin-2 receptor (IL-2R) and related cytokines were systematically detected. Results: (1) There was no significant difference in the levels of CD3, CD4 and CD4 / CD8 in peripheral blood T cell subsets in children with seizure and the normal controls, but CD8 in seizures was higher than that in normal controls (P <0. (P <0.01); (2) The levels of soluble interleukin 2 receptor (sIL-2R) and IgE in children with euthyroidism were significantly higher than those in eutopic and remission children (P <0.01) ); (3) Immunoelectron microscopy showed that the expression of IL-2R on the surface of lymphocytes in children with attack was higher than that of normal control (P <0.05). (2) The levels of sIL-2R and IgE in plasma are closely related to the changes of asthma, which can be used as clinical Indicators of asthma surveillance.