本文采用索氏提取法提取出了山丹叶中的黄酮类化合物;对其中黄酮的种类进行了化学定性研究和紫外光谱定性研究,建立用双波长分光光度法来测定山丹叶中总黄酮含量。结果表明,山丹叶中含有黄酮类化合物;双波长分光光度法具有稳定性好,准确度高,精密度好且不受葡萄糖和果糖的干扰等优点,可用于山丹叶中总黄酮含量的测定;用该方法测得山丹叶中总黄酮含量0.505%。 Soxhlet extraction method was used to extract the flavonoids from the leaves of Shandan. Chemical characterization of the flavonoids and qualitative analysis of UV spectra were used to establish the determination of total flavonoids in the leaves of Shandan by dual wavelength spectrophotometry. . The results showed that Shandan leaves contain flavonoids; dual-wavelength spectrophotometry has the advantages of good stability, high accuracy, good precision, and is free from the interference of glucose and fructose, and can be used for the content of total flavonoids in Shandan leaves. The total flavonoid content in the leaves of Shandan was measured to be 0.505%.