日本牵牛(Phartis nil),花形大,色泽艳丽,是一种重要的极具观赏价值的园艺花卉。一方面由于日本牵牛结实率低,用种子大量繁殖比较困难;另一方面牵牛属植物是研究开花机理的绝好材料,因此对牵牛属植物进行组织培养意义极其重大。由于这种植物离体再生困难,组织培养基础差,用其子叶、茎尖、幼叶和胚轴离体培养形成胚性愈伤组织和球形及心形胚较容易,却难以进一步发育成熟。以日本牵牛叶片为外植体进行组织培养以期通过组织培养 Japanese Phartis nil, with large flower shape and beautiful color, is an important ornamental gardening flower. On the one hand, because of the low seed-setting rate of Japanese bullfighting, it is more difficult to breed in large quantities with the seeds. On the other hand, the morning glory plant is an excellent material for studying the flowering mechanism. Therefore, tissue culture of the plant is of great significance. Due to the difficulty of regeneration in vitro and the poor foundation of tissue culture, it is easier to form embryogenic calli and globular and heart-shaped embryos with in vitro cultured cotyledons, shoot tips, young leaves and hypocotyls, but it is difficult to further mature. Japanese fetus leaves as explants for tissue culture in order to pass tissue culture