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目的:探讨电针对脑缺血再灌注损伤小鼠脑组织氧化应激的影响，并探明其潜在机制。方法:将45只雄性C57BL/6小鼠随机分为假手术组、模型组、电针组，采用线栓法制备小鼠脑缺血再灌注模型。电针组选取百会穴、膈俞穴、肾俞穴进行针刺治疗，每天1次，连续治疗7 d。各组小鼠进行神经功能缺损评分，HE染色观察脑组织损伤情况，试剂盒检测脑匀浆液中活性氧（ROS）、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）的水平，免疫组化法检测脑内Kelch样环氧氯丙烷相关蛋白-1（Keap-1）、核因子E2相关因子（Nrf2）、抗氧化反应元件（ARE）蛋白的表达。结果:电针治疗后，脑缺血再灌注损伤小鼠神经功能缺损评分与治疗前相比显著降低[（2.95±0.53）vs（1.43±0.39），n P<0.05]，神经元病理损伤明显改善，与模型组相比ROS、MDA水平下降[（110.71±16.92）vs（67.91±10.31）,（11.06±2.33）vs（6.29±0.57）,n P<0.05]，SOD、GSH水平上升[（18.27±3.14）vs（25.71±2.77）,（4.20±0.58）vs（6.27±0.81），n P<0.05]，Keap-1阳性表达降低[（0.623±0.087）vs（0.504±0.056），n P<0.05]，Nrf2和ARE阳性表达显著增加[（0.260±0.031）vs（0.571±0.066）,（0.384±0.039）vs（0.611±0.071）,n P<0.05）。n 结论:电针能通过激活Keap-1/Nrf2/ARE信号通路调节脑组织氧化应激因子水平，改善脑缺血再灌注损伤小鼠神经损伤行为。“,”Objective:To investigate the effect of electroacupuncture on oxidative stress in brain tissue of mice with cerebral ischemia-reperfusion injury and to explore its underlying mechanism.Methods:Forty-five male C57BL/6 mice were randomly divided into sham operation group, a model group, and an electroacupuncture group. A mouse model of cerebral ischemia and reperfusion was prepared by the thread plug method. In the electroacupuncture group, Baihui, Geshu, and Shenshu were selected for acupuncture treatment once a day for 7 consecutive days. HE staining was used to observe the overall damage of brain tissue. The kit detected oxidative stress factor reactive oxygen species (ROS) and superoxide dismutase (SOD) , malondialdehyde (MDA) , and glutathione (GSH) levels. The expression of Kelch-like epichlorohydrin-related protein-1 (Keap-1) , nuclear factor E2-related factor (Nrf2) , and antioxidant response element (ARE) protein in the brain was detected by immunohistochemistry.Results:After electroacupuncture treatment, the neurological deficit scores of mice with cerebral ischemia-reperfusion injury were significantly reduced (n P<0.05) . Pathological phenomena were improved to some extent. The levels of ROS and MDA decreased (n P<0.05) , the levels of SOD and GSH increased (n P<0.05) , the positive expression of Keap-1 decreased (n P<0.05) , and the positive expression of Nrf2 and ARE increased significantly (n P<0.05) .n Conclusion:Electroacupuncture can regulate the level of oxidative stress factor in the brain tissue by activating the Keap-1/Nrf2/ARE signaling pathway, and improve the nerve injury behavior in mice with cerebral ischemia-reperfusion injury.