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目的:探讨中药长必安(简称长必安)抗人大肠癌细胞的作用机制。方法:采用血清药理学方法,设长必安低、中、高浓度组,氟脲嘧啶(5-FU)作为阳性对照组和联合用药(长必安加5-FU)组,与我所自建的两株人大肠癌细胞系,观察了中药长必安的抗肠癌作用。结果:长必安对肠癌细胞有抑制生长和杀伤作用,其程度随长必安浓度的增高而增强。当长必安与5-FU 联合用药时对大肠癌细胞的杀伤作用更强,显示了长必安协同并增强了5-FU 的抗肿瘤作用,长必安主要对G_2+M 期细胞有阻滞作用,并能诱导细胞凋亡。结论:长必安对肠癌细胞有抑制生长和杀伤作用,长必安能协同并增强5-FU 的抗肿瘤作用。
OBJECTIVE: To explore the mechanism of anti-human colorectal cancer cells treated by Changbi’an (abbreviated as Changbi’an). METHODS: Serum pharmacological methods were used to establish a low-, medium-, and high-concentration Changbi’an group and 5-fluorouracil (5-FU) as a positive control group and a combination drug (Chang Bian plus 5-FU) group. Two human colorectal cancer cell lines were built, and the anti-cancer effect of Changbian was observed. RESULTS: Changbi’an inhibited the growth and killing of colon cancer cells, and its degree increased with the increase of the concentration of bethanechol. When combined with 5-FU, Changbiyan has a stronger killing effect on colorectal cancer cells, demonstrating that Changbi’an synergistically enhances the anti-tumor effect of 5-FU, and that Changbi’an mainly blocks G_2+M cells. Hysteresis, and can induce apoptosis. Conclusion: Changbi’an can inhibit the growth and killing of colorectal cancer cells. Changbi’an can synergistically enhance the anti-tumor effect of 5-FU.