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迄今已发现的人乳头瘤病毒（ＨＰＶ） 型已超过７７ 型，其中至少有３０ 个型与泌尿生殖道肿瘤有关。所以ＨＰＶ 的检测与分型对于泌尿生殖道肿瘤的病因和预后将是非常重要的。ＨＰＶ有很大的异质性，故用常规的诊断技术来测定未知标本中的ＨＰＶ 基因型有一定困难。为此，我们应用了反向点杂交法（ＲＤＢ） 来检测ＨＰＶ 分型。即用７ 种序列特异性寡核苷酸探针分别对应７ 型ＨＰＶ（ＨＰＶ６Ｂ、１１ 、１６ 、１８ 、３１ 、３３ 、３５） ，这些探针被合成后依次固定在一条尼龙膜上形成７ 个点，再与样品ＤＮＡ序列的ＰＣＲ产物进行杂交。结果７ 型ＨＰＶ 中任一型阳性都可以在一条尼龙膜上鉴别出来。我们收集来自广州各大医院的标本，共计１７７ 例（ 除２０ 例食道癌标本来自河南省外） ，用ＲＤＢ来检测ＨＰＶ 和分型。结果如下：子宫颈癌８６ 例，其中鳞癌６８ 例，ＨＰＶ 阳性５９ 例（８６ ．７６ ％ ） ；腺癌１８ 例，ＨＰＶ 阳性８ 例（４４ ．４４ ％ ）；尖锐湿疣４２ 例，ＨＰＶ 阳性３９ 例（９２ ．８６ ％ ） ；慢性宫颈炎２９例，ＨＰＶ阳性１ 例（３．４５ ％ ） ；食道癌２０ 例，ＨＰＶ 阳性７ 例（３５ ．００ ％ ） 。
Hitherto, human papillomavirus (HPV) types have been found to be more than 77, of which at least 30 are associated with urogenital tumors. Therefore, the detection and typing of HPV genitourinary tract cancer causes and prognosis will be very important. HPV has great heterogeneity, so using conventional diagnostic techniques to determine the HPV genotype in unknown samples have some difficulties. To this end, we applied reverse dot blot (RDB) to detect HPV typing. That is, seven types of sequence-specific oligonucleotide probes corresponding to HPV type 7 (HPV6B, 11, 16, 18, 31, 33, 35) were used. These probes were synthesized and sequentially immobilized on a nylon membrane to form seven Point and hybridize with the PCR product of the sample DNA sequence. Results of any type 7 HPV positive can be identified in a nylon membrane. We collected samples from major hospitals in Guangzhou, a total of 177 cases (except for 20 cases of esophageal cancer specimens from outside Henan Province), using RDB to detect HPV and typing. The results were as follows: There were 86 cases of cervical cancer, including 68 cases of squamous cell carcinoma, 59 cases of HPV positive (86.76%), 18 cases of adenocarcinoma, 8 cases of HPV positive (44.44%), 42 cases of genital warts, (92.86%), chronic cervicitis in 29 cases, HPV positive in 1 case (3.45%), esophageal cancer in 20 cases and HPV positive in 7 cases (35.00%).