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该文旨在探索一种适用于中药炒制品DNA快速提取的方法。以氢氧化钠,1%PVP40和1%Triton X-100配制成碱裂解缓冲液,Tris-HCl为中和液,经加热裂解和中和2步提取不同炒制方法制备的中药炮制品的DNA,选择2种方法对DNA进行纯化,并以纯化后DNA作为模板利用通用引物进行PCR扩增。结果表明优化碱裂解法可简单快速的提取出药材DNA,槐米炒制品DNA质量浓度为(420.61±123.91)g·L-1,且使用5%Chelex-100树脂纯化可以提高DNA提取浓度。研究结果证明优化碱裂解法适用于中药炒制品的DNA快速提取。
This paper aims to explore a method suitable for rapid extraction of DNA from Chinese speculation products. Preparation of alkaline lysis buffer with sodium hydroxide, 1% PVP40 and 1% Triton X-100, Tris-HCl as neutralization solution, heating pyrolysis and neutralization , Two kinds of methods were selected to purify the DNA, and the purified DNA was used as a template to carry out PCR amplification using universal primers. The results showed that the optimized alkaline lysis method could extract the DNA of the medicinal materials easily and rapidly. The DNA concentration of the pseudo-fried Sophorus japonicus was (420.61 ± 123.91) g · L-1, and the DNA extraction concentration could be increased by using 5% Chelex-100 resin. The results show that the optimized alkaline lysis method is suitable for rapid DNA extraction of Chinese speculation products.