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目的研究丹参成分丹参酮ⅡA、丹参素、丹参多酚酸盐、原儿茶醛对系统性硬皮病(SSc)成纤维细胞增殖和Ⅰ型、Ⅲ型前胶原 mRNA 合成的影响程度。方法培养来自 SSc 患者及正常对照皮肤的或纤维细胞各5株四甲基偶氮唑盐微量酶反应比色法测定丹参酮ⅡA、丹参素、丹参多酚酸盐、原儿茶醛对成纤维细胞增殖的影响,逆转录-聚合酶链反应测定硬皮病组和正常对照组以及各药物对硬皮病组的成纤维细胞Ⅰ型和Ⅲ型前胶原 mRNA 合成的影响。结果丹参酮ⅡA、丹参素、丹参多酚酸盐和原儿茶醛都对硬皮病成纤维细胞的增殖具有抑制作用(均 P<0.01),丹参酮ⅡA作用最强。4种成分都对成纤维细胞Ⅰ型和Ⅲ型胶原 mRNA 基因表达具有抑制作用(均 P<0.01),丹参酮ⅡA最强,可以使Ⅰ型前胶原 mRNA 减少47%,Ⅲ型前胶原 mRNA 减少45%。结论 4种丹参成分中丹参酮ⅡA对系统性硬皮病成纤维细胞增殖和Ⅰ、Ⅲ型前胶原 mRNA 的表达抑制作用最强,具有较强的抗纤维化作用。
Objective To study the effects of salvia miltiorrhiza tanshinone IIA, danshensu, salvianolate, protocatechuic aldehyde on the proliferation of systemic scleroderma (SSc) fibroblasts and the synthesis of type I and type III procollagen mRNA. METHODS Culture of five methyltetrazolium salts from SSc patients and normal control skin or five fibroblasts. Trace enzyme reaction colorimetric method for the determination of tanshinone IIA, danshensu, salvianolate, protocatechuic aldehyde on fibroblasts Effects of proliferation, reverse transcriptase-polymerase chain reaction assay scleroderma group and normal control group and each drug on scleroderma fibroblasts type I and III procollagen mRNA synthesis. Results Tanshinone IIA, danshensu, salvianolate and protocatechuic aldehyde all had inhibitory effects on the proliferation of scleroderma fibroblasts (all P<0.01), and tanshinone IIA had the strongest effect. All four components had inhibitory effects on fibroblasts type I and type III collagen mRNA expression (all P<0.01). Tanshinone IIA was the strongest, which reduced Type I procollagen mRNA by 47% and type III procollagen mRNA by 45. %. Conclusion Tanshinone IIA has the strongest anti-fibrotic effect on the proliferation of systemic scleroderma fibroblasts and the expression of type I and type III procollagen mRNA in four kinds of salvia miltiorrhiza.