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目的:建立鄂西北地区苍术药材HPLC指纹图谱,为苍术药材质量控制及品种鉴定提供依据。方法:采用HPLC法,以Diamonsil2#C18(250 mm×4.6 mm,5μm)色谱柱,Diamonsil预柱,流动相为乙腈-水,梯度洗脱,流速:1.0 ml·min-1,检测波长:340 nm。结果:14批苍术药材特征图谱共检出13个分离度良好的共有峰;将13个共有峰进行聚类分析,鄂西北地区苍术聚为两大类。结论:该方法简便、快捷,可作为苍术药材质量控制的有利手段。
OBJECTIVE: To establish HPLC fingerprints of herb medicines in the northwestern Hubei Province and provide the basis for the quality control and variety identification of the herb medicines. Methods: Diamonsil2 # C18 (250 mm × 4.6 mm, 5 μm) column and Diamonsil precolumn were used. The mobile phase consisted of acetonitrile-water with gradient elution at a flow rate of 1.0 ml · min- nm. Results: Thirteen common peaks with good resolution were detected in 14 batches of medicinal herbs. Cluster analysis was performed on 13 common peaks, and herbaceous plants in northwestern Hubei Province were clustered into two groups. Conclusion: The method is simple and rapid, and can be used as a favorable method for the quality control of Atractylodes.