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在过去华西1号（HX－1）液有效保存肝脏24小时，保存胰腺48小时的基础上，以高渗枸椽酸盐（HC－WCU）液为对照，采用狗自体肾移植模型观察其对狗肾脏保存效果。实验采用本地杂种狗20只，体重8～15kg，雌雄不限，随机分成①对照组（HC－WCU）；②实验组（HX－1液组），各组又根据保存时间不同分为48，72小时两个亚组，每亚组动物各5只。切取狗左侧肾脏灌注后低温保存48，72小时行自体异位肾移植。同时切取右侧肾，观察移植术后狗血肌酐，存活率，形态学检查。实验发现1，HC－WCU液48小时组术后血肌酐峰值为642．60±158．60μmol／L，72小时组术后血肌酐峰值为686．20±154．04μmol／L。HX－1液48小时组术后血肌酐峰值为448．32±36．90μmol／L，72小时组术后血肌酐峰值为524±109．38μmol／L。2．HC－WCU肾保存液保存48，72小时移植存活率为80％和40％。HX－1液保存48，72小时移植存活率为100％和60％。3存活12天两组形态学无明显差异，HC－WCU液组死于肾衰的肾组织损害明显重于HX－1液组，尤以线粒体为甚。结论：HX－1液对狗肾脏的保存效果优于HC－WCU液，其有效保存时间达72小时，保存机制可能是防止细胞水肿，减轻细胞酸中毒，减少能量消耗，抗氧自由基，保护血管内皮细胞等。它作为腹部多器官保?
In the past, Huaxi No. 1 (HX-1) effectively preserved the liver for 24 hours, and stored pancreas for 48 hours. The hypertonic citrate (HC-WCU) solution was used as a control and the dog autologous kidney transplantation model was used to observe its effects. Dog kidney preservation effect. Twenty local mongrel dogs, weighing 8 to 15 kg, male or female, randomly divided into a control group (HC-WCU); 2 experimental group (HX-1 fluid group), each group is divided into 48 according to different preservation time. 72 hours in two subgroups, 5 in each subgroup. The left kidney of the dog was sacrificed after cryopreservation for 48 and 72 hours, and autotransplantation was performed. At the same time, the right kidney was taken and the dog’s blood creatinine, survival rate and morphological examination were observed. The experimental results showed that the peak value of serum creatinine was 642.60±158.60μmol/L in HC-WCU 48 hours group, and the peak value of serum creatinine was 686.20±154.04μmol/L in 72 hours group. The peak value of serum creatinine was 448.32±36.90μmol/L in 48 hours of HX-1 solution, and 524±109.38μmol/L in 72 hours. 2. The HC-WCU kidney preservation solution was preserved for 48, 72 hours and the graft survival rate was 80% and 40%. The HX-1 solution was stored for 48 hours and the 72-hour graft survival rate was 100% and 60%. 3 There was no significant difference in morphology between the two groups after 12 days of survival. Renal tissue damage in the HC-WCU fluid group that died of renal failure was significantly heavier than that in the HX-1 fluid group, especially in the mitochondria. Conclusion: The preservation effect of HX-1 solution on dog kidney is better than that of HC-WCU solution. Its effective storage time is up to 72 hours. The storage mechanism may be to prevent cell edema, reduce cell acidosis, reduce energy consumption, resist free radicals, and protect Vascular endothelial cells. Does it serve as a multiple organ protection for the abdomen?