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为观察卵巢摘除后“延髓内脏带”内神经元的FOS表达,本文将20只成年雌性大鼠随机分为四组:(1)卵巢摘除+棉籽油组:摘除双侧卵巢后,皮下注射棉籽油(1ml/kg/d);(2)卵巢摘除+雌性激素组:皮下注射苯甲酸雌二醇(10μg/d);(3)卵巢摘除+雄性激素组:皮下注射丙酸睾丸酮(睾因,130μg/d);(4)手术但不摘除卵巢+棉籽油组。所有动物成活6周后处死,取延髓切片进行抗FOS蛋白和抗酪氨酸羟化酶的免疫组化反应。结果在第4组的“延髓内脏带”出现许多FOS阳性神经元,其中40%为FOS/酪氨酸羟化酶双重反应阳性;第2组的FOS阳性神经元次之;第3组则更少;最少者为第1组。说明“延髓内脏带”内神经元(特别是酪氨酸羟化酶阳性神经元)对体内性激素的变化起反应。
In order to observe the FOS expression of neurons in the medullary visceral zone after ovariectomy, 20 adult female rats were randomly divided into four groups: (1) ovariectomy + cottonseed oil group: cottonseed (2) ovariectomy + estrogen group: subcutaneous injection of estradiol benzoate (10μg / d); (3) ovariectomy + androgenic group: subcutaneous injection of testosterone propionate , 130μg / d); (4) surgery but not ovary + cottonseed oil group. All animals were sacrificed 6 weeks after their survival, and paraffin sections were obtained for immunohistochemical reaction of anti-FOS protein and anti-tyrosine hydroxylase. Results There were many FOS-positive neurons in the medullary visceral zone of group 4, 40% of which were positive for FOS / tyrosine hydroxylase double reaction; the second was FOS positive neurons; the third was more Less; the least for the first group. Description “medullary visceral zone” neurons (especially tyrosine hydroxylase-positive neurons) on the changes in sex hormones response.