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目的研究补肾通络法对实验性慢性非细菌性前列腺炎(CAP)动物模型前炎性基因表达的影响。方法60只Wistar大鼠随机分为正常对照组,模型对照组,前列通利汤高、低剂量组及阳性药物对照组;前列通利汤高、低剂量组及阳性药物对照组分别于造模后45天连续给药1个月,观察各组大鼠前列腺组织的整体、光镜、电镜病理形态学以及炎性基因表达产物肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)和诱导型一氧化氮合成酶(iNOs)的变化。结果前列通利汤高剂量组CAP大鼠血清或前列腺组织的TNF-α、IL-1β、IL-2、iNOs趋于正常,组织学及电镜检测出的炎性病理变化明显减轻或恢复正常,与模型对照组比较差异有统计学意义。结论以“补肾通络”为指导原则的前列通利汤能够通过抑制炎性基因的表达,调节免疫功能,从而对实验性CAP大鼠模型产生治疗作用。
Objective To study the effect of Bushen Tongluo method on the expression of pro-inflammatory genes in animal models of chronic non-bacterial prostatitis (CAP). Methods Sixty Wistar rats were randomly divided into normal control group, model control group, Qianlie Tongli Decoction high and low dose group and positive drug control group; Qianlie Tongli Decoction high and low dose group and positive drug control group were separately After continuous dosing for one month for 45 days, the overall prostate tissue, light microscopy, electron microscopic pathology, and inflammatory gene expression products of tumor necrosis factor-α (TNF-α) and interleukin-1β (pronounced as inflammatory cytokines) were observed in each group. Changes in IL-1β), interleukin-2 (IL-2), and inducible nitric oxide synthase (iNOs). Results The serum concentration of TNF-α, IL-1β, IL-2, and iNOs in serum or prostate tissue of CAP rats with high concentration of Qianlie Tongli Decoction tended to be normal, and the inflammatory pathological changes detected by histology and electron microscopy were significantly reduced or returned to normal. Compared with the model control group, the difference was statistically significant. Conclusion Qianlie Tongli Decoction, which is based on “Bushen Tongluo” as a guideline, can regulate the immune function by inhibiting the expression of inflammatory genes, and thus has a therapeutic effect on the experimental CAP rat model.