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小麦高温抗条锈性是一种在相对高温下表达的非小种专化抗病性,以典型高温抗条锈品种小偃6号为试材,研究了高温抗条锈性表达与蛋白质合成的关系。小偃 6 号小麦叶片接种条锈菌(Pucciniastriiform is)CY29 小种后,常温(14~16 ℃)培养,寄主呈亲和反应,显症后移入高温(21~22 ℃)下继续培养,诱导了高温抗锈性,表现不亲和反应。蛋白质体内合成试验发现接种苗移入高温下处理24 h 后,接种叶中标记氨基酸掺入率分别增至未经高温处理的接种叶的1.51 倍和高温处理的未接种叶的1.42 倍。Poly(A+ )-RNA 体外翻译进一步证明高温处理导致接种叶出现编码6 种新多肽的m RNA,其分子量分别为69.8,47.44,44.6,41.7,40.7 和35.5 kD,表明高温抗条锈性的表达与基因活化有关。
The stripe rust resistance of wheat at high temperature is a non-racespecific disease resistance expressed at relatively high temperature. Using the typical high-temperature resistant stripe rust cultivar Xiaoyan 6 as test material, the expression of high-temperature resistance to stripe rust and protein synthesis Relationship. Inoculation of Pucciniastriiform is CY29 races, Xiaoyan6 wheat leaves were cultured at room temperature (14 ~ 16 ℃), the host was in an affinity reaction, the disease was moved to high temperature (21 ~ 22 ℃) High temperature rust resistance, the performance of unaffinity reaction. The results of in vivo synthetic experiments showed that the incorporation rates of labeled amino acids in inoculated leaves increased 1.51 times and 1.52 times respectively in non-inoculated leaves after high temperature treatment Times In vitro translation of Poly (A +) -RNA further demonstrated that high temperature treatment led to the presence of m RNA encoding six new polypeptides in the inoculated leaves, with molecular weights of 69.8, 47.44, 44.6, 41.7, 40.7 and 35, respectively .5 kD, indicating that the expression of high temperature resistance to stripe rust and gene activation.