论文部分内容阅读
目的探讨人脐血源性间充质干细胞(mesenchymal stem cells derived from umbilical cord blood,UCB-MSCs)经成骨诱导分化后,对树突状细胞(dendritic cells,DCs)的分化、成熟及免疫功能的影响。方法对UCB-MSCs通过细胞形态、表面标记及成骨诱导分化能力进行鉴定;在外周血单核细胞培养体系中加入GM-CSF+IL-4+TNF-α,刺激DCs诱导分化及成熟;收集与成骨诱导分化前后UCB-MSCs共培养的DCs,流式细胞仪检测DCs免疫表型的表达情况;将成熟DCs作为刺激因素,外周血淋巴细胞作为反应细胞,3H-TdR标记β液体闪烁计数仪检测与成骨诱导分化前后UCB-MSCs共培养后,DCs刺激淋巴细胞增殖能力的变化。结果人UCB-MSCs成骨诱导分化后能抑制DCs表面CD40、CD80、CD83、CD86和MHC-II的表达,上调CD14的表达;DCs具有明显刺激淋巴细胞增殖的功能,而UCB-MSCs成骨诱导分化后能显著抑制DCs刺激的淋巴细胞增殖。结论成骨诱导分化的UCB-MSCs在体外可抑制同种异体DCs的分化、成熟及免疫功能,为UCB-MSCs作为同种异体源性种子细胞在骨组织工程中的应用提供了依据。
Objective To investigate the differentiation, maturation and immune function of dendritic cells (DCs) induced by osteogenic differentiation of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) Impact. Methods UCB-MSCs were identified by cell morphology, surface labeling and differentiation into osteogenic cells. GM-CSF + IL-4 + TNF-α was added to the culture system of peripheral blood mononuclear cells to induce differentiation and maturation of DCs. DCs were cultured with UCB-MSCs before and after osteogenic differentiation. Flow cytometry was used to detect the immunophenotype of DCs. Mature DCs were used as stimulating factors and peripheral blood lymphocytes as reactive cells. 3H-TdR labeled β-scintillation counter After co-culture of UCB-MSCs with UCB-MSCs before and after osteogenic differentiation, DCs stimulated the proliferation of lymphocytes. Results After osteogenic differentiation, human UCB-MSCs could inhibit the expression of CD40, CD80, CD83, CD86 and MHC-II on the surface of DCs and upregulate the expression of CD14. DCs could significantly stimulate the proliferation of lymphocytes, while UCB- Differentiation can significantly inhibit DCs stimulated lymphocyte proliferation. Conclusion The osteogenic differentiation-induced UCB-MSCs can inhibit the differentiation, maturation and immune function of allogeneic DCs in vitro and provide the basis for the application of UCB-MSCs as allogenic seed cells in bone tissue engineering.