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目的:探讨血管内皮生长因子(VEGF)936C/T基因多态性及其等位基因与重度子痫前期遗传易感性的相关关系。方法:采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)分别检测84例重度子痫前期患者和71例正常对照组的VEGF 936C/T基因多态性突变位点的基因型。结果:重度子痫前期患者中VEGF 936CT、TT基因型高于正常对照组,VEGF 936CC基因型低于正常对照组,差异有统计学意义(P<0.05);重度子痫前期组VEGF 936C等位基因低于正常对照组,VEGF 936T等位基因高于正常对照组,差异有统计学意义(P<0.05)。调整混杂因素后,正常对照组、重度子痫前期组人群中携带CT、TT基因型组发生重度子痫前期的危险性是CC基因型组的2.732倍。结论:携带VEGF 936CT、TT基因型者患重度子痫前期的危险性增加,VEGF 936C/T基因多态性与重度子痫前期的发生具有相关性。
Objective: To investigate the relationship between the polymorphism of vascular endothelial growth factor (VEGF) 936C / T gene and its genetic susceptibility to severe preeclampsia. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the gene mutation of VEGF 936C / T polymorphism in 84 patients with severe preeclampsia and 71 normal controls type. Results: The genotypes of VEGF 936CT and TT in patients with severe preeclampsia were significantly higher than those in controls and the genotypes of VEGF 936CC were lower than those in controls (P <0.05). The levels of VEGF 936C in severe preeclampsia The gene was lower than the normal control group, VEGF 936T allele was higher than the normal control group, the difference was statistically significant (P <0.05). After adjusting the confounding factors, the risk of severe preeclampsia in CT and TT genotypes in normal control group and severe preeclampsia group was 2.732 times of that in CC genotype group. CONCLUSION: The risk of severe preeclampsia is increased in patients with VEGF 936CT and TT genotypes, and the association between VEGF 936C / T polymorphism and severe preeclampsia.