论文部分内容阅读
目的 分析端粒长度及 DCC基因 m RNA表达在大肠癌及腺瘤发生发展中的作用。方法 采用 Sourthern印迹杂交及 RT- PCR技术 ,分别检测 46例大肠腺瘤及 6 2例大肠癌组织中的端粒限制性片段 (TRF)长度及 DCC m RNA表达状态 ,并观察它们与肿瘤临床病理的关系。结果 在大肠癌及大肠腺瘤中 ,TRF长度较正常组织明显缩短 ,其缩短者占 5 3.2 %和 41.3% ,而延长者仅占 6 .5 %和 4.4% ,结肠癌的TRF长度也较直肠癌的 TRF长度明显缩短。 DCC m RNA表达缺失率在大肠癌及大肠腺瘤中则分别达6 2 .9%和 34 .8% ,显著高于正常组织 (1.6 % ) ;同时 ,大肠癌患者的平均 TRF长度还随患者年龄的增长而缩短 ,DCC m RNA表达缺失率则随肿瘤的分化程度下降及临床阶段的进展而升高 ,但 DCC m RNA表达缺失与 TRF长度缩短在大肠癌中未表现出明显的相关性。结论 端粒缩短与 DCC m RNA表达缺失可能是大肠腺瘤恶变及大肠癌形成过程中较具特征表现的生物学异常行为。
Objective To analyze the role of telomere length and mRNA expression of DCC gene in the development of colorectal cancer and adenoma. METHODS: Southern blot hybridization and RT-PCR were used to detect the length of telomere restriction fragment (TRF) and the expression of DCC mRNA in 46 cases of colorectal adenoma and 62 cases of colorectal cancer, and observe their clinicopathological features. Relationship. Results In colorectal cancer and colorectal adenomas, the length of TRF was significantly shorter than that of normal tissue, accounting for 53.2% and 41.3% of the shortened length. However, the length of the extension was only 6.5% and 4.4%. The TRF length of colon cancer was also higher than that of the rectum. The TRF length of cancer is significantly shorter. The rate of DCC m RNA absent in colorectal cancer and colorectal adenoma was 6.2% and 34.8%, respectively, which was significantly higher than that of normal tissue (1.6%). Meanwhile, the average TRF length of patients with colorectal cancer also followed the patient’s As the age increases, the rate of DCC m RNA expression loss decreases with the degree of tumor differentiation and the progression of the clinical stage. However, the lack of DCC mRNA expression and shortened TRF length do not show significant correlation in colorectal cancer. Conclusion Telomere shortening and the loss of DC mRNA expression may be biological abnormalities in the malignant transformation of colorectal adenoma and the formation of colorectal cancer.