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OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q(Hop Q) mediates host-pathogen interactions; Hop Q genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against Hop Q genotypes and H. pylori cytotoxin-associated gene A(Cag A).METHODS: DNA was extracted by polymerase chain reaction of the Hop Q genotypes(i.e., type 1, type 2 and Cag A) from 115 H. pylori strains. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis.RESULTS: C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% ± 3.7%, 13.8% ± 3.6%, respectively, against Hop Q type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against Hop Q type 2. C. longa(93%), C. annum(89%) and A. esculentus(75%) had better anti-adhesive activity against H. pylori with Hop Q type 1 compared to Hop Q type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum(14.7% ± 4.1%), A. esculentus(12.3% ± 4.1%) and Z. officinale(8.4% ± 2.8%) had an anti-adhesion effect against Cag A-positive H. pylori strains compared to Cag A-negative strains.
OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (Hop Q) mediates host-pathogen interactions; Hop Q genotypes 1 and 2 are found associated with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against Hop Q genotypes and H. pylori cytotoxin-associated gene A (Cag A). METHODS: DNA was extracted by polymerase chain reaction of the Hop Q genotypes (ie, type 1, type 2 and Cag A) from 115 H. pylori strain. The effect of the extracts from selected dietary ingredients was determined using a gastric adenocarcinoma cell line and a quantitative DNA fragmentation assay. The anti-adhesive effect of these extracts on H. pylori was tested using an anti-adhesion analysis .RESULTS: C. annum, C. longa and A. esculentus showed prominent anti-adhesion effects with resultant values of 17.3% ± 2.9%, 14.6% Against Hop Q type 1 and 13.1% ± 1.7%, 12.1% ± 2%, 11.1% ± 1.6%, respectively, against Hop Q type 2. C. longa (93% , C. annum (89%) and A. esculentus (75%) had better anti-adhesive activity against H. pylori with Hop Q type 1 compared to Hop Q type 2 with respective values of 70%, 64% and 51%. Extracts of C. annum (14.7% ± 4.1%), A. esculentus (12.3% ± 4.1%) and Z. officinale (8.4% ± 2.8%) had an anti-adhesion effect against Cag A-positive H. pylori to Cag A-negative