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合成了一种新型沙丁胺醇抗原,并以此建立酶联免疫分析法(ELISA)。利用4-氨基苯甲酸作为偶联剂使沙丁胺醇与载体蛋白连接得到免疫抗原,免疫家兔得到沙丁胺醇多克隆抗体。以克伦特罗-卵蛋白代替包被抗原所建立的ELISA法对沙丁胺醇的半数抑制浓度(IC50)为8.97ng·mL?1,该抗体检测克伦特罗时有较强的交叉反应(107%),有望同时测定食物中的克伦特罗和沙丁胺醇残留。用该法测定猪肝中的沙丁胺醇加标量,回收率在70%～99%,批内差<13.3%,批间差<14.3%。利用该法可快速、灵敏地检测沙丁胺醇的药残量,为最终开发商品化的酶联免疫试剂盒打下良好的基础。
A novel albuterol antigen was synthesized and an enzyme-linked immunosorbent assay (ELISA) was developed. Using 4-aminobenzoic acid as a coupling agent, the albuterol is connected with the carrier protein to obtain the immune antigen, and the rabbit is immunized to obtain the salbutamol polyclonal antibody. The IC50 value of albuterol was 8.97 ng · mL -1 by ELISA with clenbuterol-ovalbumin instead of coated antigen. The antibody had a strong cross-reactivity when detecting clenbuterol (107 %), It is expected to simultaneously determine the residual clenbuterol and salbutamol in food. The method was used to determine salbutamol in pig liver. The recoveries ranged from 70% to 99%. The intra-assay difference was <13.3% and the inter-assay difference was <14.3%. The method can be used to detect the salbutamol fast and sensitively and lay a good foundation for the final development of a commercial enzyme immunoassay kit.