植物组织病理学研究是寄主与病原物相互关系的一个重要方面,其目的是揭示在亲和与非亲和状况下病原物侵染过程的组织学特征,以及植物组织的病变特征。植物组织病理学研究的方法目前广泛采用的是整叶棉蓝染色技术和Rohringer的整叶荧光染色技术。前一技术的染色一般难以掌握,观察效果往往不佳,尽管荧光染色方法可以克服这些缺点,但尚不能观察到寄主细胞中的病原结构,而且染色程序复杂。近年来,微分干涉相差显微镜技术在植物组 Plant Histopathology is an important aspect of the relationship between hosts and pathogens. The purpose of this study is to reveal the histological characteristics of pathogens and the pathological changes of plant tissues under both affinity and non-affinity conditions. Plant histopathology methods are currently widely used is the whole leaf cotton blue dyeing technology and Rohringer whole leaf fluorescence staining technology. The staining of the former technique is generally difficult to grasp and the observed effect is often poor. Although the fluorescent staining method can overcome these shortcomings, the pathogen structure in host cells can not be observed and the staining procedure is complicated. In recent years, differential interference phase contrast microscopy in plant groups