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为探讨HBV侵入PBMC后对乙型病毒性肝炎患者细胞免疫功能的影响,本文应用PCR技术和生物素—链霉亲合素(BSA)系统分别检测97例乙肝患者的外周血单核细胞(PBMC)内HBV—DNA和CO+3、CO+4、CO+8、CO+4/CO+8及经PHA诱导前后膜白介素—2受体(mIL—2R)的表达水平。结果显示乙型病毒性肝炎患者T细胞亚群及mIL—2R水平与正常对照相比较,均显著降低(P<0.01)。PBMC内HBV—DNA阳性组与阴性组相比较,CO+3、CO+4百分率降低、CD+8百分率增高、CO+4/CO+8比值下降及mIL—2R表达水平低下,二组差别有高度显著性(P<0.01)。说明乙型病毒性肝炎患者细胞免疫功能明显低下,HBV感染PBMC后可引起乙肝患者的细胞免疫功能紊乱
In order to investigate the effect of HBV infection on cellular immunity in patients with viral hepatitis B, 97 PBMCs (PBMCs) were detected by PCR and biotin-streptavidin (BSA) ), HBV-DNA and CO + 3, CO + 4, CO + 8, CO + 4 / CO + 8 and membrane interleukin-2receptor (mIL-2R) The results showed that the levels of T lymphocyte subsets and mIL-2R in patients with viral hepatitis B were significantly lower than those in normal controls (P <0.01). Compared with negative group, the percentage of CO + 3 and CO + 4, the percentage of CD + 8, the decrease of CO + 4 / CO + 8 and the expression of mIL-2R in PBMC were significantly lower than those in the negative group (P <0.01) . That patients with viral hepatitis B cellular immune function was significantly lower, HBV infection of PBMC can cause hepatitis B patients with cellular immune dysfunction