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目的探讨干扰素调节因子4结合蛋白(interferon regulatory factor-4 binding protein,IBP)能否影响结肠癌细胞上皮间质转化(epithelial to mesenchymal transition,EMT)的发生,观察IBP在肿瘤细胞增殖、迁移和侵袭中作用。方法干预结肠癌细胞中IBP的表达,构建IBP过表达的HT-29-IBP细胞株与IBP沉默的HCT116-sh IBP细胞株;干预IBP表达后,采用Western blot检测结肠癌细胞EMT相关分子的蛋白表达;Transwell实验检测结肠癌细胞迁移、侵袭能力的改变;MTT实验检测结肠癌细胞增殖能力的改变。结果 HT-29-IBP细胞中上皮相关分子E-cadherin和ZO-1表达水平降低,间质相关分子Fibronectin表达升高,细胞增殖、迁移和侵袭能力增强(P<0.05);HCT116-sh IBP细胞则E-cadherin、ZO-1表达水平升高,Fibronectin及Snail表达降低,细胞增殖、迁移和侵袭能力降低(P<0.05)。结论 IBP通过调节EMT促进结肠癌细胞增殖、迁移和侵袭。
Objective To investigate whether interferon regulatory factor-4 binding protein (IBP) can affect the development of epithelial to mesenchymal transition (EMT) in human colon cancer cells and to observe the effect of IBP on the proliferation, migration, Invasion role. Methods The IBP expression in colon cancer cells was intervened and the IBP over-expressed HT-29-IBP cell line and IBP-silenced HCT116-sh IBP cell line were constructed. After intervention of IBP expression, Western blot was used to detect the expression of EMT-related protein Transwell assay was used to detect the migration and invasion ability of colon cancer cells. MTT assay was used to detect the proliferation of colon cancer cells. Results The expression of epithelial related molecules E-cadherin and ZO-1 in HT-29-IBP cells decreased, the expression of fibroblastectin increased, and the ability of cell proliferation, migration and invasion increased (P <0.05) The expression of E-cadherin and ZO-1 increased, the expression of Fibronectin and Snail decreased, and the ability of cell proliferation, migration and invasion decreased (P <0.05). Conclusion IBP can promote the proliferation, migration and invasion of colon cancer cells through regulating EMT.