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目的 :观察异搏定 (verapamil ,Ver)对非p -gp高表达细胞株的增敏及耐药逆转作用。方法 :使用二甲氧唑黄比色法 (XTT法 )检测药物敏感性 ,观察Ver对鬼臼乙叉甙 (etoposide ,VP16)的增敏及耐药逆转作用。结果 :单独Ver对HL 60和HL 60 /Adr细胞株的IC50 分别为 2 6.13mg·L-1和 3 9.0 0mg·L-1,单独VP 16对HL 60细胞的IC50 为 2 47.17μg·L-1,而加用Ver 5mg·L-1预处理 1h后 ,VP16对HL 60细胞的IC50 值下降为原来的 1/1.73 ;单独VP 16对HL -60 /Adr细胞的IC50 为872 .97μg·L-1,而加用Ver 5mg·L-1预处理 1h后 ,IC50 下降至 2 0 8.45 μg·L-1,为原来的 1/4.19,与VP16对HL 60敏感株的IC50 接近。结论 :Ver对非p gp高表达细胞株具有增敏及耐药逆转作用
OBJECTIVE: To observe the sensitization and reversal of drug resistance of verapamil (Ver) to non-p-gp highly expressed cell lines. Methods: The drug sensitivity was detected by XTT method. The sensitization of Ver to etoposide (VP16) and the reversal of drug resistance were observed. Results: The IC50 of verotine alone on HL 60 and HL 60 / Adr cell lines were 2 6.13 mg · L-1 and 9.0 0 mg · L-1, respectively. The IC50 of VP 16 against HL 60 cells alone was 2 47.17 μg · L- 1, while the pretreatment with Ver 5mg · L-1 for 1h, the IC50 value of VP16 on HL 60 cells was reduced to 1 / 1.73; the IC50 of VP16 alone on HL-60 / Adr cells was 872.97μg · L -1, while the pretreatment with Ver 5mg · L-1 for 1h, the IC50 decreased to 20.45 μg · L-1, which was 1 / 4.19, which was close to that of VP16 to HL 60-sensitive strains. Conclusion: Ver has sensitization and reversal of drug resistance to non-p gp highly expressed cell lines