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采用HPLC法对60份甘蓝材料球叶中的硫甙组分和含量进行了研究,共检测到8种硫甙成分,包含5种脂肪族硫甙(3-甲基硫氧丙基硫甙、2-羟基-3-丁烯基硫甙、4-甲基亚磺酰丁基硫甙、2-丙烯基硫甙和3-丁烯基硫甙),3种吲哚族硫甙(4-羟基-3-吲哚-甲基硫甙、3-吲哚-甲基硫甙、4-甲氧基-3-吲哚-甲基硫甙).3-甲基硫氧丙基硫甙和3-吲哚-甲基硫甙是甘蓝硫甙的主要组分,约占总硫甙含量的59%.总硫甙含量在6.822～45.379μmol/g(DW)之间,总脂肪族硫甙含量在2.362～34.036μmol/g(DW)之间,总吲哚族硫甙含量在2.382～24.110μmol/g(DW)之间.
The glucosinolate components and contents in 60 leaves of cabbage were studied by HPLC. Eight kinds of glucosinolate components were detected, including 5 kinds of aliphatic glucosinolate (3-methylthioxypropylthioglycoside, 2-hydroxy-3-butenyl glucosinolate, 4-methyl sulfinyl butyl glucosinolate, 2-propenyl glucosinolate and 3-butenyl glucosinolate), 3 indoles glucosinolates Hydroxy-3-indolylmethylthioside, 3-indolylmethylthioside, 4-methoxy-3-indolylmethylthioside), 3-methylthioxypropylthioside and 3-Indole-methylthioglycoside is the main component of the total glucosinolates, accounting for about 59% of the total glucosinolate content, total glucosinolate content of 6.822 ~ 45.379μmol / g (DW) The content of indole glucosinolate was between 2.362 ~ 34.036μmol / g (DW), and the total indole glucosinolate content was between 2.382 ~ 24.110μmol / g (DW).