论文部分内容阅读
目的:探讨宫颈癌组织中是否存在γδT细胞以及γδT细胞在体外抗肿瘤作用。方法:免疫组织化学法检测10例宫颈癌组织中γδT细胞的数量,固相抗体包被法体外扩增出特异性的γδT细胞后用流式细胞仪检测其纯度,乳酸脱氢酶释放实验检测γδT细胞对宫颈癌细胞株SiHa、HeLa细胞的杀伤能力。结果:免疫组化结果显示人宫颈癌组织中肿瘤浸润γδT细胞阳性染色较癌旁组织显著增强,该细胞通过固相抗体包被法体外扩增30d之后用流式细胞仪检测细胞表面TCRγδ的阳性率高达(91.2±1.2)%,乳酸脱氢酶释放实验显示体外扩增的γδT细胞能够杀伤宫颈癌细胞株SiHa、HeLa细胞,并且其杀伤效应随效应细胞/靶细胞的比例增高而增强。结论:宫颈癌组织中存在γδT细胞,该细胞在体外对宫颈癌细胞具有杀伤作用,其杀伤效应随效应细胞/靶细胞的比例增高而增强。
Objective: To investigate the existence of γδT cells and γδT cells in cervical cancer in vitro anti-tumor effect. Methods: Immunohistochemical method was used to detect the number of γδT cells in 10 cases of cervical cancer. The purity of γδT cells was detected by flow cytometry after the solid phase antibody coated with γδT cells. The lactate dehydrogenase release assay Cytotoxicity of γδT cells to cervical cancer cell lines SiHa and HeLa. Results: Immunohistochemistry showed that the positive staining of tumor-infiltrated γδT cells in human cervical cancer tissues was significantly enhanced compared with that in adjacent non-cancerous tissues. After the cells were solid-phase antibody-coated and expanded for 30 days, the cell surface TCRγδ was detected by flow cytometry Lactate dehydrogenase release assay showed that γδT cells expanded in vitro could kill cervical cancer cell lines SiHa and HeLa, and the killing effect increased with the ratio of effector cells / target cells. Conclusion: γδT cells exist in cervical cancer tissues. The cells have cytotoxic effect on cervical cancer cells in vitro. The killing effect of γδT cells increases with the proportion of effector cells / target cells.