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目的通过分析艾滋病病毒(HIV)抗体检测不确定结果的发生特点,探讨HIV抗体不确定产生的原因以及减少HIV抗体不确定可能的措施。方法采用HIV抗体检测策略和方法,对宁夏2010-2015年HIV抗体检测数据进行回顾性分析。结果 2010-2015年,宁夏艾滋病确证中心实验室HIV抗体检测不确定者72例,随访21例,经确证阳性12例(16.7%),阴性9例(12.5%)。随访的21例中有3例进行了核酸检测,结果大于104拷贝/mL,均阳转。12例阳转样本中,Env类条带不确定的,经随访检测后诊断为阳性8例;Gag类条带不确定的,随访检测阳性4例。2例Pol类条带不确定的,随访检测后未确诊阳性。阳转患者平均间隔时间为39.8天,阳转后gp160、gp120、p66、p24出现率为100%。结论宁夏HIV抗体检测不确定结果的发生率呈逐年上升趋势,对检测结果为“不确定”患者启动核酸检测,作为辅助诊断,有利于缩短确证时间和减轻患者压力。
OBJECTIVE: To analyze the causes of the indetermination of HIV antibody and the possible measures to reduce the uncertainty of HIV antibody by analyzing the characteristics of HIV test results. Methods HIV antibody detection strategies and methods were used to analyze retrospectively the HIV antibody test data from 2010 to 2015 in Ningxia. Results From 2010 to 2015, there were 72 cases with undetermined HIV antibody test in HIV / AIDS confirmed center of Ningxia. Totally 21 cases were followed up, 12 cases (16.7%) were confirmed positive and 9 cases (12.5%) were negative. Three of the 21 cases followed up with a nucleic acid test that yielded a positive result of more than 104 copies / mL. In 12 cases of positive rotation, the Env-like bands were indefinite and 8 cases were positive after follow-up examination. The Gag-like bands were uncertain and the follow-up test was positive in 4 cases. Two cases of Pol-like bands were indefinite and were not diagnosed as positive after follow-up examination. Yang transfer average interval of 39.8 days patients, gp160, gp120, p66, p24 positive after the turn of the incidence of 100%. Conclusion The incidence of HIV antibody testing in Ningxia has been increasing year by year. Nucleic acid testing is started in patients with test results of “Uncertainty ”, which is helpful to shorten the confirmation time and relieve the pressure of patients.