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目的建立食品中黄曲霉毒素B1、B2、G1、G2的光化学衍生高效液相色谱荧光检测方法。方法用乙腈-水提取样品中的黄曲霉毒素,经多功能柱净化,以38/62的甲醇-水为流动相等度洗脱,高效液相色谱分离,光化学衍生器衍生,荧光检测器检测。结果 4种黄曲霉毒素在24 min内得到良好的基线分离,黄曲霉毒素B1、G1的检出限为0.2μg/kg,黄曲霉毒素B2、G2的检出限为0.1μg/kg。在1.0~5.0μg/kg添加范围内,加标回收率为84.0%~94.6%,相对标准偏差为3.4%~6.5%。结论该方法灵敏度高,操作简便,准确可靠,重现性好,适于对食品中黄曲霉毒素的日常检测。
Objective To establish a method for the determination of aflatoxins B1, B2, G1, G2 in food by high performance liquid chromatography with fluorescence detection. Methods The aflatoxins in the samples were extracted with acetonitrile - water and purified by a multi - functional column. The mobile phase consisted of 38/62 methanol - water as eluent, and separated by high performance liquid chromatography (HPLC) and derivatized with photochemical derivatization and fluorescence detector. Results The four aflatoxins showed good baseline separation within 24 min. The detection limit of aflatoxin B1 and G1 was 0.2 μg / kg and the detection limit of aflatoxin B2 and G2 was 0.1 μg / kg. The spiked recoveries ranged from 84.0% to 94.6% and the relative standard deviations (RSDs) ranged from 3.4% to 6.5% in the range of 1.0 ~ 5.0 μg / kg. Conclusion The method is sensitive, simple, accurate, reliable and reproducible. It is suitable for the routine detection of aflatoxins in food.