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近年来文献中提到的逆行荧光神经元标记物已有多种,但这些标记物均存在某些缺点和不足。我们发现国产酸性荧光染料达旦黄(简称TY,化学纯,批号781002,亭新,上海)有沿轴突逆行运送至神经细胞并似无扩散和损伤神经组织的缺点。本研究用大白鼠27只.将5—10％TY作单侧尾壳核、舌肌、颈段脊髓注射。在上述3只单侧尾壳核或/和舌注射TY动物中,其另一侧注射10—20％樱草黄(Pr)。存活期4小时至21天后灌注10％福马林或能诱发儿茶酚胺荧光的Faglu液,取脑,冰切,有些尾壳核注射部位作石蜡切片,焦油紫染色。用Ni-
There are many retrograde fluorescent neuron markers mentioned in the literature in recent years, but these markers all have some shortcomings and deficiencies. We found that the domestic acid fluorescent dyestuff (TY, chemically pure, lot number 781002, Ting new, Shanghai) along the retrograde axonal delivery to nerve cells and no diffusion and damage the nervous tissue defects. In this study, 27 rats were used, and 5-10% TY was injected into the caudate putamen, tongue muscle and cervical spinal cord. In the three unilateral caudate putamen or / and tongue-injected TY animals mentioned above, 10-20% primrose (Pr) was injected on the other side. Survival of 4 hours to 21 days after perfusion with 10% Fmalin or Faglu induced fluorescence of catecholamines, brain, ice-cutting, some caudate putamen nuclear injection site for paraffin section, tar purple staining. Ni-