取小鼠脾细胞悬液,先用NH_4Cl溶解红细胞,过玻璃棉柱去除巨噬细胞,然后在兔抗小鼠Ig包被的塑料平板上进行阳性选择,可比较容易而快速地将淋巴细胞分为粘附[Ig~+(B)]细胞与非粘附(Ig~-)细胞。免疫荧光试验证明粘附细胞中97.5%是Ig~+的,只有2.2%为Thy 1.2~+。用流式细胞计和光镜进一步检查Ig~+细胞,证明是一群非常均一的细胞,其中大多数为小淋巴细胞,也 Take the mouse spleen cell suspension, first with NH_4Cl red blood cells were dissolved, the glass wool column to remove macrophages, and then coated with rabbit anti-mouse Ig plastic positive selection can be relatively easy and fast lymphocyte sub To adhere [Ig ~ + (B)] cells and non-adherent (Ig ~ -) cells. Immunofluorescence assay showed that 97.5% of adherent cells were Ig ~ + and only 2.2% were Thy 1.2 ~ +. Further examination of Ig ~ + cells using flow cytometry and light microscopy proved to be a very homogeneous group of cells, most of them small lymphocytes, and